Temo ke mohloli oa bohlokoa ka ho fetisisa mebarakeng ea lefats'e, 'me litsamaiso tsa tikoloho li tobane le liphephetso tse ngata. Tšebeliso ea manyolo a lik'hemik'hale lefatšeng ka bophara e ntse e hola 'me e bapala karolo ea bohlokoa chaing ea lijalo1. Leha ho le joalo, limela tse lengoang ka tsela ena ha li na nako e lekaneng ea ho hola le ho hola hantle ka hona ha li fumane boleng bo botle ba limela2. Ho phaella moo, metsoako e chefo e kotsi haholo e ka bokellana 'meleng oa motho le mobung3. Ka hona, ho na le tlhoko ea ho hlahisa litharollo tse mosa tikolohong le tse tšoarellang ho fokotsa tlhoko ea manyolo a lik'hemik'hale. Likokoana-hloko tse molemo e ka ba mohloli oa bohlokoa oa metsoako ea tlhaho e sebetsang ka baeloji4.
Metse ea endophytic makhasing e fapana ho latela mofuta oa semela se amohelang kapa mofuta oa genotype, mohato oa kholo ea semela, le sebopeho sa semela. 13 Lithuto tse 'maloa li tlalehile hore Azospirillum, Bacillus, Azotobacter, Pseudomonas, le Enterobacter li na le bokhoni ba hokhothalletsa kholo ea limela. 14 Ho feta moo, Bacillus le Azospirillum ke mefuta e ithutoang haholo ea PGPB mabapi le ho ntlafatsa kholo le chai ea limela. 15 Lithuto li bontšitse hore ho enta hammoho Azospirillum brasiliensis le Bradyrhizobium lijong tsa linaoa ho ka ntlafatsa chai ea poone, koro, soya le linaoa tsa kidney. 16, 17 Lithuto li bontšitse hore ho enta Salicornia ka Bacillus licheniformis le li-PGPB tse ling ho khothalletsa kholo ea limela le ho monya limatlafatsi ka tsela e kopanetsoeng. 18 Azospirillum brasiliensis Sp7 le Bacillus sphaericus UPMB10 li ntlafatsa kholo ea metso ea banana e monate. Ka ho tšoanang, peo ea fennel e thata ho e holisa ka lebaka la kholo e mpe ea limela le ho mela hanyane, haholo-holo tlas'a maemo a khatello ea komello20. Kalafo ea peo ka Pseudomonas fluorescens le Trichoderma harzianum e ntlafatsa kholo ea pele ea lipeo tsa fennel tlas'a maemo a khatello ea komello21. Bakeng sa stevia, ho entsoe lithuto ho lekola litlamorao tsa li-fungus tsa mycorrhizal le kholo ea limela tse khothalletsang rhizobacteria (PGPR) bokhoning ba sebopuoa ho hola, ho bokella li-metabolite tsa bobeli, le ho hlahisa liphatsa tsa lefutso tse amehang ho etseng lintho tse phelang. Ho ea ka Rahi et al.22, ho enta limela tse nang le li-PGPR tse fapaneng ho ntlafalitse kholo ea tsona, index ea photosynthetic, le ho bokellana ha stevioside le stevioside A. Ka lehlakoreng le leng, ho enta stevia ka rhizobia e khothalletsang kholo ea limela le li-fungus tsa arbuscular mycorrhizal ho hlohlellelitse bolelele ba semela, stevioside, liminerale le litaba tsa pigment.23 Oviedo-Pereira et al.24 ba tlalehile hore li-endophyte tse halefisang Enterobacter hormaechei H2A3 le H5A2 li ekelitse litaba tsa SG, li hlohlelletse bongata ba trichome makhasing, 'me li khothalletsa ho bokellana ha li-metabolite tse itseng ka har'a li-trichomes, empa ha lia ka tsa khothalletsa kholo ea limela;
GA3 ke e 'ngoe ea liprotheine tse kang gibberellin tsa bohlokoa ka ho fetisisa le tse sebetsang ka baeloji31. Kalafo ea kantle ea stevia ka GA3 e ka eketsa ho lelefala ha kutu le lipalesa32. Ka lehlakoreng le leng, lithuto tse ling li tlalehile hore GA3 ke ntho e susumetsang limela ho hlahisa li-metabolite tsa bobeli tse kang li-antioxidants le li-pigment, 'me hape ke mokhoa oa tšireletso33.
Likamano tsa phylogenetic tsa li-isolates mabapi le mefuta e meng ea mofuta. Linomoro tsa ho kenella ha GenBank li fanoe ka har'a masakaneng.
Mesebetsi ea Amylase, cellulase le protease e bontšoa e le lihlopha tse hlakileng ho potoloha likolone, ha matheba a masoeu ho potoloha likolone a bontša mosebetsi oa lipase. Joalokaha ho bontšitsoe ho Tafole ea 2, B. paramycoides SrAM4 e ka hlahisa li-hydrolase tsohle, ha B. paralicheniformis SrMA3 e ka hlahisa lienzyme tsohle ntle le cellulase, 'me B. licheniformis SrAM2 e hlahisa cellulase feela.
Mefuta e 'maloa ea bohlokoa ea likokoana-hloko e 'nile ea amahanngoa le keketseho ea tlhahiso ea bobeli ea metabolite limela tsa meriana le tse nkhang hamonate74. Li-antioxidants tsohle tsa enzymatic le tse seng tsa enzymatic li eketsehile haholo ho S. rebaudiana Shou-2 ha li bapisoa le taolo. Tšusumetso e ntle ea PGPB ho TPC ka raese le eona e tlalehiloe ke Chamam et al.75; Ho feta moo, liphetho tsa rona li tsamaellana le liphetho tsa TPC, TFC, le DPPH ho S. rebaudiana, e ileng ea bakoa ke ketso e kopaneng ea Piriformospora indica le Azotobacter chroococcum76. TPC le TFC77 li ne li le holimo haholo limela tsa basil tse phekoloang ka likokoana-hloko ha li bapisoa le limela tse sa phekoloang. Ho feta moo, keketseho ea li-antioxidants e ka etsahala ka mabaka a mabeli: li-enzyme tsa hydrolytic li susumetsa mekhoa ea tšireletso ea limela e susumelitsoeng ka tsela e tšoanang le likokoana-hloko tse bakang pathogenic ho fihlela semela se ikamahanya le colonization ea baktheria78. Ea bobeli, PGPB e ka sebetsa e le moqali oa tlhahiso ea metsoako ea bioactive e entsoeng ka tsela ea shikimate limela tse phahameng le likokoana-hloko 79.
Liphetho li bontšitse hore ho ne ho e-na le kamano e kopanetsoeng pakeng tsa palo ea makhasi, tlhahiso ea liphatsa tsa lefutso le tlhahiso ea SG ha mefuta e mengata e ne e entoa hammoho. Ka lehlakoreng le leng, ho entoa habeli ho ne ho le molemo ho feta ho entoa ha motho a le mong mabapi le kholo le tlhahiso ea limela.
Li-enzyme tsa hydrolytic li ile tsa fumanoa ka mor'a ho enta libaktheria holim'a agar medium e nang le substrate e bontšang le ho ikatisa ka 28 °C matsatsi a 2-5. Kamora ho phuthela libaktheria holim'a starch agar medium, ts'ebetso ea amylase e ile ea fumanoa ka ho sebelisa tharollo ea iodine 100. Ts'ebetso ea cellulase e ile ea fumanoa ka ho sebelisa 0.2% aqueous Congo red reagent ho latela mokhoa oa Kianngam et al. 101. Ts'ebetso ea protease e ile ea bonoa ka libaka tse hlakileng tse potolohileng likolone tse koahetsoeng holim'a skim milk agar medium joalo ka ha ho hlalositsoe ke Cui et al. 102. Ka lehlakoreng le leng, lipase 100 e ile ea fumanoa ka mor'a ho enta holim'a Tween agar medium.
Nako ea poso: Pherekhong-06-2025