Kea leboha ha u etetse Nature.com.Mofuta oa sebatli oo u o sebelisang o na le tšehetso e fokolang ea CSS.Bakeng sa liphetho tse ntle ka ho fetisisa, re khothaletsa hore o sebelise mofuta o mocha oa sebatli sa hau (kapa o tima Compatibility Mode ho Internet Explorer).Khabareng, ho netefatsa ts'ehetso e tsoelang pele, re bonts'a sebaka sena ntle le setaele kapa JavaScript.
Limela tsa makhasi a khabisitsoeng tse nang le ponahalo e tlokomang li bohlokoa haholo.Tsela e 'ngoe ea ho finyella sena ke ho sebelisabalaoli ba kholo ea limelajoalo ka lisebelisoa tsa taolo ea kholo ea limela.Boithuto bona bo entsoe ho Schefflera dwarf (semela sa makhasi a mekhabiso) se phekotsoeng ka li-sprays tsa makhasi.asiti ea gibberellikle hormone ea benzyladenine ka kamoreng ea sethopo e nang le tsamaiso ea nosetso ea moholi.Hormone e ile ea fafatsoa makhasi a schefflera e nyane maemong a 0, 100 le 200 mg/l ka mekhahlelo e meraro matsatsing a mang le a mang a 15.Teko e ile ea etsoa ka mokhoa o hlophisitsoeng ka mokhoa o hlophisitsoeng ka ho feletseng o nang le liphetolelo tse 'nè.Motsoako oa gibberellic acid le benzyladenine ka motsoako oa 200 mg / l o bile le phello e kholo palo ea makhasi, sebaka sa makhasi le bophahamo ba semela.Kalafo ena e boetse e hlahisitse litaba tse phahameng ka ho fetisisa tsa li-pigment tsa photosynthetic.Ho phaella moo, litekanyo tse phahameng ka ho fetisisa tsa lik'habohaedreite tse qhibilihang le ho fokotsa tsoekere li ile tsa hlokomeloa ka benzyladenine ho 100 le 200 mg / L le gibberellic acid + benzyladenine ho 200 mg / L.Tshekatsheko ya kgatelopele ya Stepwise e bontshitse gore boalo jwa modi e ne e le phetogo ya ntlha ya go tsenngwa mo mmotlolong, e tlhalosa 44% ya pharologano.Phapang e latelang e ne e le boima ba metso e mecha, 'me mohlala oa bivariate o hlalosa 63% ea phapang ea palo ea makhasi.Litholoana tse ntle ka ho fetisisa palo ea makhasi li ile tsa hlahisoa ke boima ba metso e ncha (0.43), e neng e amana hantle le palo ea makhasi (0.47).Liphello li bontšitse hore gibberellic acid le benzyladenine ka bongata ba 200 mg / l e ntlafalitse haholo kholo ea morphological, chlorophyll le carotenoid synthesis ea Liriodendron tulipifera, le ho fokotsa litaba tsa tsoekere le lik'habohaedreite tse qhibilihang.
Schefflera arborescens (Hayata) Merr ke semela se lulang se le setala se khabisang sa lelapa la Araliaceae, se tsoaletsoeng Chaena le Taiwan1.Hangata semela sena se lengoa joalo ka semela sa ntlo, empa ke semela se le seng feela se ka holang maemong a joalo.Makhasi a na le lipampitšana tse 5 ho isa ho tse 16, tse bolelele ba 10-20 cm2.Dwarf Schefflera e rekisoa ka bongata selemo se seng le se seng, empa mekhoa ea sejoale-joale ea ho lema ha e sebelisoe hangata.Ka hona, ts'ebeliso ea balaoli ba kholo ea limela e le lisebelisoa tse sebetsang tsa taolo ho ntlafatsa kholo le tlhahiso e tsitsitseng ea lihlahisoa tsa temo ea limela e hloka tlhokomelo e eketsehileng.Kajeno, ts'ebeliso ea balaoli ba kholo ea limela e eketsehile haholo3,4,5.Gibberellic acid ke taolo ea kholo ea limela e ka eketsang chai ea limela6.E 'ngoe ea litlamorao tsa eona tse tsebahalang ke ts'usumetso ea kholo ea limela, ho kenyeletsoa ho lelefala ha kutu le metso le sebaka sa makhasi a eketseha7.Phello ea bohlokoa ka ho fetisisa ea gibberellins ke keketseho ea bophahamo ba kutu ka lebaka la ho lelefatsa ha li-internode.Ho fafatsa ka foliar ea gibberellins limela tse nyenyane tse sa khoneng ho hlahisa li-gibberellins ho fella ka ho eketseha ha kutu le bolelele ba semela8.Ho fafatsa ka foliar ea lipalesa le makhasi a nang le gibberellic acid ka motsoako oa 500 mg/l ho ka eketsa bolelele ba semela, palo, bophara le bolelele ba makhasi9.Ho tlalehoa hore li-gibberellins li susumetsa kholo ea limela tse fapaneng tsa makhasi a sephara10.Elongation ea stem e ile ea bonoa ho Scots pine (Pinussylvestris) le spruce e tšoeu (Piceaglauca) ha makhasi a ne a fafatsoa ka gibberellic acid11.
Phuputso e 'ngoe e ile ea hlahloba liphello tsa li-regulator tse tharo tsa kholo ea limela tsa cytokinin ho thehoa ha lekala la lateral ho Lily officinalis.bend Liteko li ile tsa etsoa hoetla le selemo ho ithuta liphello tsa nako.Liphello li bontšitse hore kinetin, benzyladenine le 2-prenyladenine ha lia ka tsa ama ho thehoa ha makala a eketsehileng.Leha ho le joalo, 500 ppm benzyladenine e ile ea etsa hore ho thehoe makala a 12,2 le 8,2 ka hoetla le liteko tsa selemo, ka ho latellana, ha ho bapisoa le 4,9 le 3,9 makala a limela tsa taolo.Liphuputso li bontšitse hore liphekolo tsa lehlabula li sebetsa ho feta tsa mariha12.Tekong e 'ngoe, Peace Lily var.Limela tsa Tassone li ne li phekoloa ka 0, 250 le 500 ppm benzyladenine ka lipitsa tsa bophara ba 10 cm.Liphello li bontšitse hore phekolo ea mobu e ekelitse haholo palo ea makhasi a eketsehileng ha a bapisoa le limela tse laolang le tse nang le benzyladenine.Makhasi a macha a tlatsetso a ile a bonoa libeke tse 'ne ka mor'a kalafo, mme tlhahiso e phahameng ea makhasi e ile ea bonoa libeke tse robeli kamora kalafo.Ka mor'a libeke tse 20 ka mor'a ho phekoloa, limela tse hloekisitsoeng mobu li ne li e-na le phaello e fokolang ho feta limela tse hloekisitsoeng pele13.Ho tlalehiloe hore benzyladenine ka motsoako oa 20 mg / L e ka eketsa haholo bophahamo ba semela le palo ea makhasi ho Croton 14. Ka likhahla tsa calla, benzyladenine ka bongata ba 500 ppm e entse hore ho be le keketseho ea palo ea makala, ha palo e ntse e eketseha. ea makala e ne e le bonyane sehlopheng sa taolo15.Sepheo sa phuputso ena e ne e le ho batlisisa ho fafatsoa ha foliar ea gibberellic acid le benzyladenine ho ntlafatsa kholo ea Schefflera dwarfa, semela sa mekhabiso.Litaolo tsena tsa kholo ea limela li ka thusa balemi ba khoebo ho rera tlhahiso e nepahetseng selemo ho pota.Ha ho liphuputso tse entsoeng ho ntlafatsa kholo ea Liriodendron tulipifera.
Boithuto bona bo entsoe setsing sa lipatlisiso tsa semela sa kahare sa Univesithi ea Islamic Azad e Jiloft, Iran.Ho ile ha lokisoa li-transplants tsa metso e tšoanang tsa schefflera e nyenyane ea 25 ± 5 cm (e phatlalalitsoeng likhoeli tse tšeletseng pele ho teko) 'me e jaloa ka lipitsa.Pitsa ke polasetiki, e ntšo, ka bophara ba 20 cm le bolelele ba 30 cm16.
Mokhoa oa setso thutong ena e ne e le motsoako oa peat, humus, lehlabathe le hlatsoitsoeng le lekhapetla la raese ka karolelano ea 1: 1: 1: 1 (ka molumo)16.Beha moalo oa majoana botlaaseng ba pitsa bakeng sa ho ntša metsi.Karolelano ea mocheso oa motšehare le bosiu ka har'a sethopo qetellong ea selemo le lehlabula e ne e le 32±2°C le 28±2°C, ka ho latellana.Mongobo o itseng o fihla ho >70%.Sebelisa tsamaiso ea moholi bakeng sa ho nosetsa.Ka karolelano, limela li nosetsoa makhetlo a 12 ka letsatsi.Nakong ea hoetla le lehlabula, nako ea ho nosetsa e 'ngoe le e' ngoe ke metsotso e 8, nako ea ho nosetsa ke hora e le 'ngoe.Limela ka mokhoa o ts'oanang li ile tsa lengoa ka makhetlo a mane, libeke tse 2, 4, 6 le 8 ka mor'a ho jala, ka tharollo ea micronutrient (Ghoncheh Co., Iran) ka motsoako oa 3 ppm le ho nosetsoa ka 100 ml ea tharollo nako le nako.Tharollo ea limatlafatsi e na le N 8 ppm, P 4 ppm, K 5 ppm le trace elements Fe, Pb, Zn, Mn, Mo le B.
Likhakanyo tse tharo tsa asiti ea gibberellic le molaoli oa kholo ea limela benzyladenine (e rekiloeng ho Sigma) li lokisitsoe ka 0, 100 le 200 mg/L mme tsa fafatsoa holim'a likhahla tsa limela ka mekhahlelo e meraro ka nako ea matsatsi a 1517.Pakeng tsa 20 (0.1%) (e rekiloeng ho Sigma) e ne e sebelisoa ka tharollo ho eketsa nako ea eona ea nako e telele le tekanyo ea ho monya.Hoseng ka meso, fafatsa lihomone ho li-buds le makhasi a Liriodendron tulipifera u sebelisa sefafatsi.Limela li fafatsoa ka metsi a hloekisitsoeng.
Bophahamo ba semela, bophara ba kutu, sebaka sa makhasi, litaba tsa chlorophyll, palo ea li-internode, bolelele ba makala a bobeli, palo ea makala a bobeli, bophahamo ba motso, bolelele ba lekhasi, motso, kutu le lintho tse omeletseng tse ncha, likahare tsa li-photosynthetic pigments (chlorophyll). a, chlorophyll b) Kakaretso ea chlorophyll, carotenoids, palo eohle ea li-pigments), ho fokotsa tsoekere le lik'habohaedreite tse qhibilihang li ile tsa lekanyetsoa liphekolong tse fapaneng.
Boleng ba chlorophyll ba mahlaku a manyenyane bo ile ba lekanngwa matsatsi a 180 ka mora ho fafatsa ka ho sebedisa chlorophyll meter (Spad CL-01) ho tloha ka 9:30 ho isa ho 10 hoseng (ka lebaka la botjha ba makgasi).Ho feta moo, sebaka sa makhasi se lekantsoe matsatsi a 180 kamora ho fafatsa.Beha makhasi a mararo ho tloha holimo, bohareng le tlase ho kutu ho tloha pitseng ka 'ngoe.Makhasi ana a sebelisoa e le lithempleite pampiring ea A4 'me sephetho sa sephetho sea khaoloa.Boima le bokaholimo ba leqephe le le leng la pampiri ea A4 le tsona li ile tsa lekanngoa.Ebe sebaka sa makhasi a stenciled se baloa ho sebelisoa litekanyo.Ho feta moo, bophahamo ba motso bo ile ba khethoa ka ho sebelisa silinda e koetlisitsoeng.Boima ba lekhasi bo omeletseng, boima bo omeletseng ba kutu, boima bo omeletseng ba motso, le boima bo felletseng ba sampole ka 'ngoe li lekantsoe ka ho omisoa ka ontong ka 72°C bakeng sa lihora tse 48.
Litaba tsa chlorophyll le carotenoids li ile tsa lekanngoa ka mokhoa oa Lichtenthaler18.Ho etsa sena, 0.1 g ea makhasi a macha e ne e siloa ka seretse sa porcelain se nang le 15 ml ea 80% ea acetone, 'me ka mor'a ho sefa, boholo ba bona ba mahlo bo ile ba lekanyetsoa ho sebelisoa spectrophotometer ho wavelengths ea 663.2, 646.8 le 470 nm.Lekanya sesebelisoa u sebelisa 80% ea acetone.Bala palo ea li-pigment tsa photosynthetic u sebelisa equation e latelang:
Har'a tsona, Chl a, Chl b, Chl T le Car li emela chlorophyll a, chlorophyll b, chlorophyll eohle le carotenoids, ka ho latellana.Liphetho li hlahisoa ka semela sa mg/ml.
Ho fokotsa tsoekere ho ile ha lekanyetsoa ho sebelisoa mokhoa oa Somogy19.Ho etsa sena, 0,02 g ea letlobo la limela e siloa ka seretse sa porcelain ka 10 ml ea metsi a silafetseng ebe e tšeloa ka khalase e nyenyane.Chesa khalase ho fihlela e bela ebe u sefa litaba u sebelisa pampiri ea sefe ea Whatman No. 1 ho fumana moqomo oa semela.Fetisetsa 2 ml ea karolo e 'ngoe le e' ngoe ka har'a tube ea teko 'me u kenye 2 ml ea tharollo ea koporo ea sulfate.Koahela tube ea teko ka boea ba k'hothone 'me u chese ka bateng ea metsi ka 100 ° C metsotso e 20.Mothating ona, Cu2+ e fetoleloa ho Cu2O ka ho fokotsa aldehyde monosaccharide mme 'mala oa salmon (terracotta) o bonahala ka tlase ho tube ea teko.Ka mor'a hore tube ea teko e pholile, eketsa 2 ml ea phosphomolybdic acid 'me ho tla hlaha' mala o moputsoa.Hlakola tube ka matla ho fihlela 'mala o aroloa ka tsela e tšoanang ho pholletsa le tube.Bala ho monya ha tharollo ka 600 nm u sebelisa spectrophotometer.
Lekola khatello ea tsoekere ea ho fokotsa tsoekere u sebelisa curve e tloaelehileng.Tekanyo ea lik'habohaedreite tse qhibilihang e ne e khethoa ke mokhoa oa Fales20.Ho etsa sena, 0.1 g ea limela e ne e kopantsoe le 2.5 ml ea 80% ea ethanol ka 90 ° C bakeng sa metsotso e 60 (mehato e 'meli ea metsotso e 30 ka' ngoe) ho ntša lik'habohaedreite tse qhibilihang.Ka mor'a moo, mokelikeli oa hloekisoa ebe joala bo fetoha mouoane.Sephetho se hlahisoang se qhibiliha ka 2.5 ml ea metsi a hloekisitsoeng.Tšela 200 ml ea sampuli e 'ngoe le e' ngoe ka har'a tube ea teko 'me u kenye 5 ml ea athrone indicator.Motsoako o ne o kenngoa ka ho hlapa metsi ka 90 ° C bakeng sa metsotso e 17, 'me ka mor'a ho pholile, ho kenngoa ha eona ho ne ho khethoa ho 625 nm.
Teko e ne e le teko ea factorial e thehiloeng ho moralo o hlophisitsoeng ka botlalo o nang le liphetolelo tse 'ne.Ts'ebetso ea PROC UNIVARIATE e sebelisetsoa ho hlahloba maemo a tloaelehileng a kabo ea data pele ho tlhahlobo ea phapang.Tlhahlobo ea lipalo-palo e qalile ka tlhahlobo e hlalosang ea lipalo-palo ho utloisisa boleng ba data e tala e bokeletsoeng.Lipalo li etselitsoe ho nolofatsa le ho hatella li-data tse kholo ho etsa hore ho be bonolo ho li toloka.Ka mor'a moo, ho ile ha etsoa litlhahlobo tse rarahaneng haholoanyane.Teko ea Duncan e entsoe ho sebelisoa software ea SPSS (version 24; IBM Corporation, Armonk, NY, USA) ho bala likarolo tse bolelang le liphoso tsa liteko ho fumana phapang lipakeng tsa sete ea data.Teko e ngata ea Duncan (DMRT) e ne e sebelisetsoa ho khetholla phapang pakeng tsa mekhoa e boemong ba bohlokoa ba (0.05 ≤ p).Pearson coefficient coefficient ( r ) e ne e baloa ho sebelisoa SPSS software (version 26; IBM Corp., Armonk, NY, USA) ho lekola kamano pakeng tsa lipara tse fapaneng tsa liparamente.Ntle le moo, tlhahlobo ea tatellano ea mela e entsoe ho sebelisoa software ea SPSS (v.26) ho bolela esale pele boleng ba mefuta ea selemo sa pele e ipapisitseng le boleng ba mefuta ea selemo sa bobeli.Ka lehlakoreng le leng, ho ile ha etsoa tlhahlobo ea butle-butle ka p <0.01 ho khetholla litšobotsi tse susumetsang makhasi a manyenyane a schefflera.Tlhahlobo ea litsela e ile ea etsoa ho fumana liphello tse tobileng le tse sa tobang tsa tšobotsi e 'ngoe le e' ngoe ea mohlala (ho itšetlehile ka litšobotsi tse hlalosang phapang hantle).Lipalo tsohle tse ka holimo (tloaelo ea ho ajoa ha data, coefficient e bonolo ea ho hokahanya, ho fokotseha ha mohato le tlhahlobo ea tsela) li entsoe ho sebelisoa software ea SPSS V.26.
Lisampole tse khethiloeng tsa limela tse lenngoeng li ne li tsamaellana le litataiso tse amehang tsa setheo, tsa naha le tsa machabeng le melao ea lehae ea Iran.
Letlapa la 1 le bontša lipalo-palo tse hlalosang moelelo, ho kheloha ho tloaelehileng, bonyane, boholo, mefuta-futa, le phenotypic coefficient of variation (CV) bakeng sa litšobotsi tse sa tšoaneng.Har'a lipalo-palo tsena, CV e lumella papiso ea litšobotsi hobane ha e na moeli.Ho fokotsa tsoekere (40.39%), boima bo omileng ba metso (37.32%), boima ba metso e ncha (37.30%), karolelano ea tsoekere ho tsoekere (30.20%) le bophahamo ba metso (30%) ke tsona tse phahameng ka ho fetisisa.le litaba tsa chlorophyll (9.88%).) mme sebaka sa mahlaku se na le index e phahameng ka ho fetisisa (11.77%) mme se na le boleng bo tlase ba CV.Letlapa la 1 le bontša hore boima ba 'mele bo feletseng bo na le mefuta e phahameng ka ho fetisisa.Leha ho le joalo, tšobotsi ena ha e na CV e phahameng ka ho fetisisa.Ka hona, metrics e se nang moeli joalo ka CV e lokela ho sebelisoa ho bapisa liphetoho tsa sebopeho.CV e phahameng e bontša phapang e kholo lipakeng tsa kalafo bakeng sa tšobotsi ena.Liphetho tsa teko ena li bonts'itse phapang e kholo lipakeng tsa kalafo e nang le tsoekere e tlase ka boima bo omeletseng ba motso, boima ba motso o mocha, karo-karolelano ea lik'habohaedreite ho tsoekere, le litšobotsi tsa bophahamo ba motso.
Liphetho tsa ANOVA li bontšitse hore ha li bapisoa le taolo, ho fafatsa ka foliar ka gibberellic acid le benzyladenine ho bile le phello e kholo bophahamong ba semela, palo ea makhasi, sebaka sa makhasi, bophahamo ba motso, bolelele ba metso, index ea chlorophyll, boima bo bocha le boima bo omileng.
Papiso ea boleng ba boleng e bonts'itse hore balaoli ba kholo ea limela ba na le phello e kholo ho bolelele ba semela le palo ea makhasi.Liphekolo tse sebetsang ka ho fetisisa e ne e le gibberellic acid ka bongata ba 200 mg / l le gibberellic acid + benzyladenine ka bongata ba 200 mg / l.Ha ho bapisoa le taolo, bolelele ba semela le palo ea makhasi e eketsehile ka linako tse 32.92 le linako tse 62.76, ka ho latellana (Letlapa la 2).
Sebaka sa makhasi se eketsehile haholo ka mefuta eohle ha se bapisoa le taolo, ka keketseho e kholo e bonoang ho 200 mg / l bakeng sa gibberellic acid, e fihlang ho 89.19 cm2.Liphello li bontšitse hore sebaka sa makhasi se eketsehile haholo ka ho eketseha ha lihlopha tsa taolo ea kholo (Letlapa la 2).
Liphekolo tsohle li ile tsa eketsa molumo oa motso le bolelele haholo ha li bapisoa le taolo.Ho kopana ha gibberellic acid + benzyladenine ho bile le phello e kholo ka ho fetisisa, ho eketsa molumo le bolelele ba motso ka halofo ho bapisoa le taolo (Letlapa la 2).
Litekanyetso tse phahameng ka ho fetisisa tsa bophara ba stem le bolelele ba internode li ile tsa bonoa taolong le gibberellic acid + benzyladenine 200 mg/l kalafo, ka ho latellana.
Lenane la chlorophyll le eketsehile ka mefuta eohle ha e bapisoa le taolo.Ntho e phahameng ka ho fetisisa ea tšobotsi ena e ile ea hlokomeloa ha e tšoaroa ka gibberellic acid + benzyladenine 200 mg / l, e neng e le 30,21% e phahameng ho feta taolo (Letlapa la 2).
Liphello li bontšitse hore phekolo e ile ea fella ka ho se tšoane ho hoholo ha li-pigment content, ho fokotseha ha tsoekere le lik'habohaedreite tse qhibilihang.
Kalafo e nang le gibberellic acid + benzyladenine e ile ea fella ka hore ho be le boholo ba li-pigment tsa photosynthetic.Letšoao lena le ne le phahame haholo ka mefuta eohle ho feta taolong.
Liphetho li bonts'itse hore liphekolo tsohle li ka eketsa litaba tsa chlorophyll tsa Schefflera dwarf.Leha ho le joalo, boleng bo phahameng ka ho fetisisa ba tšobotsi ena bo ile ba hlokomeloa kalafo ea gibberellic acid + benzyladenine, e neng e le 36,95% e phahameng ho feta taolo (Letlapa la 3).
Liphetho tsa chlorophyll b li ne li tšoana ka ho felletseng le liphetho tsa chlorophyll a, phapang feela e ne e le keketseho ea litaba tsa chlorophyll b, e neng e phahame ka 67.15% ho feta taolong (Letlapa la 3).
Kalafo e hlahisitse keketseho e kholo ea kakaretso ea chlorophyll ha e bapisoa le taolo.Kalafo ka gibberellic acid 200 mg / l + benzyladenine 100 mg / l e lebisitse ho boleng bo phahameng ka ho fetisisa ba tšobotsi ena, e neng e le 50% e phahameng ho feta taolo (Letlapa la 3).Ho ea ka liphello, ho laola le kalafo ka benzyladenine ka tekanyo ea 100 mg / l e lebisitse ho litekanyetso tse phahameng ka ho fetisisa tsa tšobotsi ena.Liriodendron tulipifera e na le boleng bo phahameng ka ho fetisisa ba carotenoids (Letlapa la 3).
Liphetho li ile tsa bontša hore ha tšoaroa ka gibberellic acid e ka ea mahloriso ea 200 mg / L, diteng tsa chlorophyll e haholo eketseha ho chlorophyll b (sa feiga. 1).
Phello ea gibberellic acid le benzyladenine ho a/b Ch.Likarolo tsa schefflera e nyane.(GA3: gibberellic acid le BA: benzyladenine).Litlhaku tse tšoanang setšoantšong ka seng li bontša hore phapang ha e bohlokoa (P <0.01).
Phello ea phekolo e 'ngoe le e' ngoe ho boima bo bocha le bo omeletseng ba patsi e nyenyane ea schefflera e ne e phahame haholo ho feta ea taolo.Gibberellic acid + benzyladenine ho 200 mg / L e ne e le phekolo e sebetsang ka ho fetisisa, e eketsang boima bo bocha ka 138,45% ha e bapisoa le taolo.Ha ho bapisoa le taolo, mekhoa eohle ea phekolo ntle le 100 mg / L benzyladenine e ile ea eketsa boima bo omileng ba semela, 'me 200 mg / L gibberellic acid + benzyladenine e ile ea fella ka boleng bo phahameng ka ho fetisisa bakeng sa tšobotsi ena (Letlapa la 4).
Boholo ba mefuta e fapaneng e fapane haholo le taolo tabeng ena, ka litekanyetso tse phahameng ka ho fetisisa tsa 100 le 200 mg / l benzyladenine le 200 mg / l gibberellic acid + benzyladenine (setšoantšo sa 2).
Tšusumetso ea gibberellic acid le benzyladenine ho karo-karolelano ea lik'habohaedreite tse qhibilihang le ho fokotsa tsoekere ho schefflera e nyane.(GA3: gibberellic acid le BA: benzyladenine).Litlhaku tse tšoanang setšoantšong ka seng ha li bontše phapang e khōlō (P <0.01).
Ho ile ha etsoa tlhahlobo ea stepwise ho fumana litšoaneleho tsa 'nete le ho utloisisa hamolemo kamano pakeng tsa mefuta e ikemetseng le nomoro ea makhasi ho Liriodendron tulipifera.Molumo oa metso e ne e le mofuta oa pele o kentsoeng mofuteng, o hlalosang 44% ea phapang.Phapang e latelang e ne e le boima ba metso e ncha, 'me mefuta ena e' meli e hlalositse 63% ea phapang ea palo ea makhasi (Letlapa la 5).
Tlhahlobo ea litsela e ile ea etsoa ho hlalosa hantle ho fokotseha ha mehato (Letlapa la 6 le Setšoantšo sa 3).Phello e ntle ka ho fetisisa palo ea makhasi e ne e amahanngoa le boima ba metso e mecha (0.43), e neng e amana hantle le palo ea makhasi (0.47).Sena se bontša hore tšobotsi ena e ama chai ka ho toba, athe phello ea eona e sa tobang ka litšobotsi tse ling e ke ke ea hlokomolohuoa, le hore tšobotsi ena e ka sebelisoa e le mokhoa oa ho khetha mananeong a ho ikatisa bakeng sa schefflera e nyenyane.Phello e tobileng ea bophahamo ba motso e ne e le mpe (−0.67).Tšusumetso ea tšobotsi ena palo ea makhasi e tobileng, tšusumetso e sa tobang ha e na thuso.Sena se bontša hore ha motso o le moholo, palo ea makhasi e fokotseha.
Setšoantšo sa 4 se bontša liphetoho ho theolelo ea mola oa metso le ho fokotsa tsoekere.Ho ea ka "regression coefficient", karolo e 'ngoe le e' ngoe e fetoha bolelele ba metso le lik'habohaedreite tse qhibilihang ho bolela hore molumo oa motso le tsoekere e fokotsehang e fetoha ka li-unit tsa 0.6019 le 0.311.
Pearson coefficient coefficient ea litšobotsi tsa kgolo e bontšoa ho Setšoantšo sa 5. Liphello li bontšitse hore palo ea makhasi le bophahamo ba semela (0.379 *) e ne e e-na le kamano e ntle ka ho fetisisa le ea bohlokoa.
'Mapa oa mocheso oa likamano pakeng tsa mefuta e fapaneng ea li-coefficients tsa kholo ea kholo.# Y Axis: 1-Index Ch., 2-Internode, 3-LAI, 4-N ea makhasi, 5-Height ea maoto, 6-Stem bophara.# Haufi le axis ea X: A - index H., B - sebaka se pakeng tsa nodes, C - LAY, D - N. ea lekhasi, E - bophahamo ba leoto la borikhoe, F - bophara ba kutu.
Pearson coefficient coefficient bakeng sa litšobotsi tse amanang le boima ba metsi e bontšoa ho Setšoantšo sa 6. Liphetho li bontša kamano pakeng tsa boima ba lekhasi le boima bo omileng ka holim'a lefatše (0.834 **), boima bo omileng kaofela (0.913 **) le boima bo omileng ba metso (0.562 *) )..Kakaretso ea boima bo omileng bo na le kamano e ntle ka ho fetesisa le ea bohlokoa ka ho fetesisa le boima ba letlobo (0.790**) le boima ba metso (0.741**).
'Mapa oa mocheso oa likamano lipakeng tsa mefuta e mecha ea coefficient ea boima ba 'mele.# Y axis: 1 - boima ba makhasi a macha, 2 - boima ba li-buds tse ncha, 3 - boima ba metso e mecha, 4 - boima ba makhasi a macha.# X-axis e emetse: A - boima ba makhasi a macha, B - boima ba leqhubu le lecha, CW - boima ba motso o mocha, D - boima bo bocha kaofela.
Li-coefficients tsa Pearson tsa correlation bakeng sa litšoaneleho tse amanang le boima bo omeletseng li bontšoa ho Setšoantšo sa 7. Liphetho li bontša hore boima ba lekhasi bo omileng, boima bo ommeng ba bud (0.848 **) le boima bo omileng kaofela (0.947 **), boima ba bud dry (0.854 **) le boima bo omileng kaofela (0.781**) bo na le litekanyetso tse phahameng ka ho fetisisa.khokahano e ntle le khokahano ea bohlokoa.
'Mapa oa mocheso oa likamano lipakeng tsa mefuta e fapaneng ea coefficient ea boima bo omeletseng.# Y axis e emela: boima ba makhasi a 1, boima bo ommeng ba 2-bud, boima bo omileng ba metso e 3, boima ba 4 kaofela.# X Axis: Boima bo omeletseng ba makhasi, B-bud e omeletseng boima ba 'mele, boima bo omileng ba motso oa CW, D-kakaretso ea boima bo omileng.
Pearson coefficient ea corelation ea thepa ea pigment e bontšoa ho Setšoantšo sa 8. Liphetho li bontša hore chlorophyll a le chlorophyll b (0.716 **), chlorophyll eohle (0.968 **) le kakaretso ea li-pigments (0.954 **);chlorophyll b le kakaretso ea chlorophyll (0.868 **) le kakaretso ea li-pigments (0.851 **);kakaretso ea chlorophyll e na le kamano e phahameng ka ho fetisisa e ntle le ea bohlokoa le kakaretso ea mebala (0.984**).
'Mapa oa mocheso oa likamano lipakeng tsa chlorophyll coefficient coefficient variables.# Lilepe tsa Y: 1- Channel a, 2- Channel.b,3 - a/b karo-karolelano, likanale tse 4.Kakaretso, 5-carotenoids, li-pigment tse 6 tsa lihlahisoa.# Lilepe tsa X: A-Ch.aB-Ch.b,C- a/b karo-karolelano, D-Ch.Kakaretso ea dikahare, E-carotenoids, F-chino ea li-pigments.
Dwarf Schefflera ke semela se tsebahalang lefatšeng ka bophara, 'me kholo ea sona le nts'etsopele ea sona e ntse e hlokomeloa haholo.Tšebeliso ea li-regulator tsa kholo ea limela e hlahisitse phapang e kholo, 'me liphekolo tsohle li eketsa bolelele ba semela ha li bapisoa le taolo.Le hoja bolelele ba semela hangata bo laoloa ka liphatsa tsa lefutso, lipatlisiso li bontša hore ts'ebeliso ea lilaoli tsa kholo ea limela e ka eketsa kapa ea fokotsa bolelele ba semela.Bophahamo ba semela le palo ea makhasi a tšoaroang ka gibberellic acid + benzyladenine 200 mg / L e ne e le eona e phahameng ka ho fetisisa, e fihla ho 109 cm le 38,25, ka ho latellana.Ho lumellana le lithuto tse fetileng (SalehiSardoei et al.52) le Spathiphyllum23, keketseho e tšoanang ea bophahamo ba semela ka lebaka la phekolo ea gibberellic acid e ile ea hlokomeloa ka marigolds a potted, albus alba21, daylilies22, daylilies, agarwood le likhahla tsa khotso.
Gibberellic acid (GA) e bapala karolo ea bohlokoa lits'ebetsong tse fapaneng tsa 'mele tsa limela.Li susumetsa karohano ea lisele, ho lelefatsa lisele, ho lelefatsa stem le ho eketseha ha boholo24.GA e susumetsa karohano ea lisele le bolelele ba li-apices le meristems25.Liphetoho tsa makhasi li boetse li kenyelletsa botenya ba lehlaka le fokotsehileng, boholo ba makhasi a manyane, le 'mala o motala o khanyang26.Liphuputso tse sebelisang lintho tse thibelang kapa tse susumetsang li bontšitse hore li-ion tsa calcium tse tsoang mehloling e ka hare li sebetsa e le manqosa a bobeli tseleng ea pontšo ea gibberellin ho sorghum corolla27.HA e eketsa bolelele ba semela ka ho hlohlelletsa motsoako oa li-enzyme tse bakang khatholoho ea lerako la sele, joalo ka XET kapa XTH, e atoloha le PME28.Sena se etsa hore lisele li hōle ha lerako la sele le phutholoha 'me metsi a kena ka seleng29.Tšebeliso ea GA7, GA3 le GA4 e ka eketsa bolelele ba kutu30,31.Asiti ea Gibberellic e baka bolelele ba kutu limeleng tse nyenyane, 'me limela tsa rosette li sitisa ho hōla ha makhasi le bolelele ba internode32.Leha ho le joalo, pele ho sethaleng sa ho ikatisa, bolelele ba stem bo eketseha ho makhetlo a 4-5 bophahamo ba eona ba pele33.Ts'ebetso ea GA biosynthesis limela e akaretsoa setšoantšong sa 9.
GA biosynthesis limeleng le maemo a endogenous bioactive GA, kemelo ea moralo ea limela (ka ho le letona) le GA biosynthesis (ka ho le letšehali).Metsu e ngotsoe ka mebala e lumellanang le mokhoa oa HA o bontšitsoeng tseleng ea biosynthetic;metsu e khubelu e bonts'a maemo a GC a fokotsehileng ka lebaka la ho lula sebakeng sa litho tsa limela, 'me metsu e ntšo e bontša ho eketseha ha maemo a GC.Limeleng tse ngata, joalo ka raese le lehapu, GA e na le boleng bo holimo botlaaseng kapa karolong e ka tlase ea lekhasi30.Ho feta moo, litlaleho tse ling li bonts'a hore litaba tsa bioactive GA li fokotseha ha makhasi a lelefala ho tloha ho base34.Maemo a nepahetseng a gibberellins maemong ana ha a tsejoe.
Balaoli ba kholo ea limela le bona ba susumetsa haholo palo le sebaka sa makhasi.Liphetho li bonts'itse hore ho eketsa bongata ba taolo ea kholo ea limela ho felletse ka keketseho e kholo sebakeng sa makhasi le palo.Benzyladenine e tlalehiloe hore e eketsa tlhahiso ea lekhasi la calla15.Ho ea ka liphello tsa thuto ena, liphekolo tsohle li ntlafalitse sebaka sa makhasi le palo.Gibberellic acid + benzyladenine e bile kalafo e sebetsang ka ho fetesisa mme e hlahisitse palo e kholo le sebaka sa makhasi.Ha u holisa schefflera e nyane ka tlung, ho ka ba le keketseho e hlokomelehang ea palo ea makhasi.
Kalafo ea GA3 e ekelitse bolelele ba internode ha e bapisoa le benzyladenine (BA) kapa ho se na phekolo ea li-hormone.Sephetho sena sea utloahala ho latela karolo ea GA ho khothaletsa kholo7.Ho hōla ha stem ho boetse ho bontšitse liphello tse tšoanang.Gibberellic acid e ile ea eketsa bolelele ba kutu empa ea fokotsa bophara ba eona.Leha ho le joalo, ts'ebeliso e kopaneng ea BA le GA3 e ile ea eketsa bolelele ba kutu.Keketseho ena e ne e phahame ha e bapisoa le limela tse tšoaroang ka BA kapa ntle le hormone.Le hoja gibberellic acid le cytokinins (CK) ka kakaretso li khothalletsa kholo ea limela, maemong a mang li na le liphello tse fapaneng le mekhoa e fapaneng35.Mohlala, tšebelisano e mpe e ile ea bonoa keketsehong ea bolelele ba hypocotyl ho limela tse tšoaroang ka GA le BA36.Ka lehlakoreng le leng, BA e ile ea eketsa molumo oa metso haholo (Letlapa la 1).Keketseho ea metso ka lebaka la BA ea kantle ho naha e tlalehiloe limela tse ngata (mohlala Dendrobium le Orchid mefuta)37,38.
Kalafo eohle ea li-hormone e ile ea eketsa palo ea makhasi a macha.Keketseho ea tlhaho sebakeng sa makhasi le bolelele ba kutu ka liphekolo tse kopaneng ke ntho e lakatsehang khoebong.Palo ea makhasi a macha ke sesupo sa bohlokoa sa kholo ea limela.Tšebeliso ea li-hormone tsa exogenous ha e so sebelisoe tlhahiso ea khoebo ea Liriodendron tulipifera.Leha ho le joalo, liphello tse khothalletsang kholo ea GA le CK, tse sebelisoang ka ho leka-lekana, li ka fana ka lintlha tse ncha tsa ho ntlafatsa temo ea semela sena.Ka ho hlakileng, phello ea synergistic ea phekolo ea BA + GA3 e ne e phahame ho feta ea GA kapa BA e tsamaisoang feela.Gibberellic acid e eketsa palo ea makhasi a macha.Ha makhasi a macha a ntse a hōla, ho eketsa palo ea makhasi a macha ho ka fokotsa kholo ea makhasi39.Ho tlalehoa hore GA e ntlafatsa ho tsamaisoa ha sucrose ho tloha litebeng ho ea ho litho tsa mohloli40,41.Ho phaella moo, ts'ebeliso e sa tloaelehang ea GA ho limela tse sa feleng e ka khothalletsa kholo ea litho tsa limela tse kang makhasi le metso, kahoo e thibela phetoho ea ho tloha ho limela ho ea ho kholo ea ho ikatisa42.
Phello ea GA ho eketseha ha limela tse omeletseng li ka hlalosoa ka ho eketseha ha photosynthesis ka lebaka la ho eketseha ha sebaka sa makhasi43.GA e tlalehiloe e baka keketseho ea sebaka sa makhasi a Maize34.Liphetho li bontšitse hore ho eketsa khatello ea BA ho 200 mg / L ho ka eketsa bolelele le palo ea makala a bobeli le bophahamo ba metso.Gibberellic acid e susumetsa mekhoa ea lisele tse kang ho susumetsa karohano ea lisele le ho lelefatsa, kahoo ho ntlafatsa kholo ea limela43.Ho phaella moo, HA e atolosa lerako la sele ka ho kenya starch ka hydrolyzing hore e be tsoekere, ka tsela eo e fokotsa matla a sele ea metsi, e leng ho etsang hore metsi a kene ka seleng 'me qetellong a lebisa ho elongation ea sele44.
Nako ea poso: Jun-11-2024