Kabo ea pula ea sehla Profenseng ea Guizhou ha e lekane, ka pula e ngata nakong ea selemo le lehlabula, empa lipeo tsa peo ea rapese li kotsing ea khatello ea komello nakong ea hoetla le mariha, e leng se amang chai haholo. Mosetareta ke sejalo se khethehileng sa peo ea oli se lengoang haholo Profenseng ea Guizhou. E na le mamello e matla ea komello 'me e ka lengoa libakeng tse lithaba. Ke mohloli o ruileng oa liphatsa tsa lefutso tse hanelang komello. Ho sibolloa ha liphatsa tsa lefutso tse hanelang komello ho bohlokoa haholo bakeng sa ntlafatso ea mefuta ea mosetareta. le boqapi mehloling ea germplasm. Lelapa la GRF le bapala karolo ea bohlokoa kholong le nts'etsopele ea limela le karabelo khatellong ea komello. Hona joale, liphatsa tsa lefutso tsa GRF li fumanoe ho Arabidopsis 2, raese (Oryza sativa) 12, peo ea rapese 13, k'hothone (Gossypium hirsutum) 14, koro (Triticum). aestivum)15, perela ea millet (Setaria italica)16 le Brassica17, empa ha ho na litlaleho tsa liphatsa tsa lefutso tsa GRF tse fumanoeng mosetareteng. Phuputsong ena, liphatsa tsa lefutso tsa lelapa la mosetareta tsa GRF li ile tsa khetholloa boemong ba genome ka bophara 'me litšobotsi tsa tsona tsa' mele le tsa lik'hemik'hale, likamano tsa ho iphetola ha lintho, homology, motifs e bolokiloeng, sebopeho sa liphatsa tsa lefutso, ho pheta-pheta ha liphatsa tsa lefutso, likarolo tsa cis le mohato oa peō (mokhahlelo oa makhasi a mane) li ile tsa hlahlojoa. Mekhoa ea tlhahiso tlas'a khatello ea komello e ile ea hlahlojoa ka botlalo ho fana ka motheo oa saense bakeng sa lithuto tse ling mabapi le mosebetsi o ka bang teng oa liphatsa tsa lefutso tsa BjGRF karabelong ea komello le ho fana ka liphatsa tsa lefutso tse ka khethoang bakeng sa ho ikatisa mosetareta o mamellang komello.
Liphatsa tsa lefutso tse mashome a mararo a metso e mene tsa BjGRF li ile tsa fumanoa ka har'a genome ea Brassica juncea ho sebelisoa lipatlisiso tse peli tsa HMMER, tseo kaofela li nang le libaka tsa QLQ le WRC. Tatellano ea CDS ea liphatsa tsa lefutso tsa BjGRF tse khethiloeng e hlahisoa Tafoleng e Tlatsetsang S1. BjGRF01–BjGRF34 li rehiloe mabitso ho latela sebaka sa tsona ho chromosome. Thepa ea fisik'hemik'hale ea lelapa lena e bontša hore bolelele ba amino acid boa fetoha haholo, ho tloha ho 261 aa (BjGRF19) ho isa ho 905 aa (BjGRF28). Ntlha ea isoelectric ea BjGRF e tloha ho 6.19 (BjGRF02) ho isa ho 9.35 (BjGRF03) ka karolelano ea 8.33, 'me 88.24% ea BjGRF ke protheine ea motheo. Bongata ba boima ba limolek'hule ba BjGRF bo boletsoeng esale pele bo tloha ho 29.82 kDa (BjGRF19) ho isa ho 102.90 kDa (BjGRF28); index ea ho se tsitse ha liprotheine tsa BjGRF e tloha ho 51.13 (BjGRF08) ho isa ho 78.24 (BjGRF19), kaofela li kholo ho feta 40, e leng se bontšang hore index ea fatty acid e tloha ho 43.65 (BjGRF01) ho isa ho 78.78 (BjGRF22), karolelano ea hydrophilicity (GRAVY) e tloha ho -1.07 (BjGRF31) ho isa ho -0.45 (BjGRF22), liprotheine tsohle tsa BjGRF tse hydrophilistic li na le boleng bo bobe ba GRAVY, e ka 'nang ea bakoa ke khaello ea hydrophobicity e bakoang ke masala. Ponelopele ea sebaka sa lisele tse ka tlas'a sele e bontšitse hore liprotheine tse 31 tse kentsoeng ka BjGRF li ka fumanoa ka har'a nucleus, BjGRF04 e ka fumanoa ka har'a peroxisomes, BjGRF25 e ka fumanoa ka har'a cytoplasm, 'me BjGRF28 e ka fumanoa ka har'a li-chloroplast (Tafole ea 1), e leng se bontšang hore li-BjGRF li ka fumanoa ka har'a nucleus 'me li bapala karolo ea bohlokoa ea taolo e le ntlha ea phetolelo.
Tlhahlobo ea phylogenetic ea malapa a GRF mefuteng e fapaneng e ka thusa ho ithuta mesebetsi ea liphatsa tsa lefutso. Ka hona, tatellano ea bolelele bo felletseng ba amino acid ea li-GRF tse 35 tsa rapeseed, turnip tse 16, raese tse 12, millet tse 10 le li-GRF tse 9 tsa Arabidopsis li ile tsa jarollwa 'me sefate sa phylogenetic sa hahuoa ho latela liphatsa tsa lefutso tse 34 tse khethiloeng tsa BjGRF (Setšoantšo sa 1). Malapa a mararo a manyenyane a na le palo e fapaneng ea litho; Li-TF tsa GRF tse 116 li arotsoe ka malapa a manyenyane a mararo a fapaneng (lihlopha tsa A~C), tse nang le 59 (50.86%), 34 (29.31%) le 23 (19.83)% ea li-GRF, ka ho latellana. Har'a tsona, litho tse 34 tsa lelapa la BjGRF li hasane ho malapa a manyenyane a 3: litho tse 13 sehlopheng sa A (38.24%), litho tse 12 sehlopheng sa B (35.29%) le litho tse 9 sehlopheng sa C (26.47%). Mokhoeng oa ho ba le polyploidization ea mosetareta, palo ea liphatsa tsa lefutso tsa BjGRF malapeng a fapaneng a fapaneng e fapane, 'me keketseho le tahlehelo ea liphatsa tsa lefutso e kanna ea ba e etsahetse. Hoa bohlokoa ho hlokomela hore ha ho na kabo ea li-GRF tsa raese le millet sehlopheng sa C, ha ho na le li-GRF tse 2 tsa raese le millet GRF e le' ngoe sehlopheng sa B, 'me boholo ba li-GRF tsa raese le millet li arotsoe ka lekala le le leng, ho bontšang hore li-BjGRF li amana haufi-ufi le li-dicot. Har'a tsona, lithuto tse tebileng ka ho fetisisa mabapi le ts'ebetso ea GRF ho Arabidopsis thaliana li fana ka motheo oa lithuto tsa ts'ebetso ea li-BjGRF.
Sefate sa mosetareta se nang le phylogenetic se kenyeletsang Brassica napus, Brassica napus, raese, nyalothe le litho tsa lelapa la Arabidopsis thaliana GRF.
Tlhahlobo ea liphatsa tsa lefutso tse pheta-phetoang lelapeng la mosetareta GRF. Mola o moputsoa ka morao o emela boloko bo lumellanang ho genome ea mosetareta, mola o mofubelu o emela lipheta-pheta tse peli tse arohaneng tsa gene ea BjGRF;
Tlhahiso ea liphatsa tsa lefutso tsa BjGRF tlas'a khatello ea komello mohatong oa bone oa lekhasi. Lintlha tsa qRT-PCR li bontšitsoe Tafoleng e Tlatsetsang S5. Liphapang tse kholo tsa lintlha li bontšoa ka litlhaku tse nyane.
Ha boemo ba leholimo ba lefats'e bo ntse bo fetoha, ho ithuta kamoo lijalo li sebetsanang le khatello ea komello le ho ntlafatsa mekhoa ea tsona ea ho mamella e se e le sehlooho se chesang sa lipatlisiso18. Kamora komello, sebopeho sa sebopeho, tlhahiso ea liphatsa tsa lefutso le lits'ebetso tsa metabolism tsa limela li tla fetoha, e leng se ka lebisang ho emisong ea photosynthesis le tšitiso ea metabolism, e amang chai le boleng ba lijalo19,20,21. Ha limela li utloa matšoao a komello, li hlahisa manģosa a bobeli joalo ka Ca2+ le phosphatidylinositol, li eketsa mahloriso a calcium ion a ka hare ho sele le ho kenya tšebetsong marang-rang a taolo a tsela ea phosphorylation ea protheine22,23. Protheine ea ho qetela e reretsoeng e ameha ka kotloloho ts'ireletsong ea sele kapa e laola tlhahiso ea liphatsa tsa lefutso tse amanang le khatello ka li-TF, e ntlafatsa mamello ea limela khatellong24,25. Kahoo, li-TF li bapala karolo ea bohlokoa ho arabeleng khatellong ea komello. Ho ea ka tatellano le thepa ea ho tlama DNA ea li-TF tse arabelang khatello ea komello, li-TF li ka aroloa ka malapa a fapaneng, joalo ka GRF, ERF, MYB, WRKY le malapa a mang26.
Lelapa la liphatsa tsa lefutso la GRF ke mofuta oa TF e ikhethileng ea limela e bapalang karolo ea bohlokoa likarolong tse fapaneng tse kang kholo, nts'etsopele, phetisetso ea matšoao le likarabelo tsa ts'ireletso ea limela27. Ho tloha ha jini ea pele ea GRF e fumanoa ho O. sativa28, liphatsa tsa lefutso tse ngata tsa GRF li se li fumanoe mefuteng e mengata 'me li bontšitsoe li ama kholo ea limela, nts'etsopele le karabelo ea khatello ea maikutlo8, 29, 30,31,32. Ka phatlalatso ea tatellano ea genome ea Brassica juncea, ho tsebahala ha lelapa la liphatsa tsa lefutso la BjGRF ho ile ha khoneha33. Thutong ena, liphatsa tsa lefutso tse 34 tsa BjGRF li ile tsa fumanoa genome eohle ea mosetareta 'me tsa rehoa BjGRF01–BjGRF34 ho latela boemo ba tsona ba chromosome. Kaofela ha tsona li na le libaka tsa QLQ le WRC tse bolokiloeng haholo. Tlhahlobo ea thepa ea fisik'hemik'hale ea fisik'hemik'hale e bontšitse hore liphapang tsa linomoro tsa amino acid le boima ba limolek'hule tsa liprotheine tsa BjGRF (ntle le BjGRF28) li ne li sa bohlokoa, ho bontša hore litho tsa lelapa la BjGRF li ka ba le mesebetsi e tšoanang. Tlhahlobo ea sebopeho sa liphatsa tsa lefutso e bontšitse hore 64.7% ea liphatsa tsa lefutso tsa BjGRF li ne li e-na le li-exons tse 4, e leng se bontšang hore sebopeho sa liphatsa tsa lefutso tsa BjGRF se bolokiloe ka mokhoa o batlang o tšoana le phetohong ea lintho, empa palo ea li-exons liphatseng tsa lefutso tsa BjGRF10, BjGRF16, BjGRP28 le BjGRF29 e kholoanyane. Lithuto li bontšitse hore ho eketsoa kapa ho tlosoa ha li-exons kapa li-intron ho ka lebisa liphapang sebopehong le ts'ebetsong ea liphatsa tsa lefutso, ka hona ho theha liphatsa tsa lefutso tse ncha34,35,36. Ka hona, re nahana hore intron ea BjGRF e lahlehile nakong ea phetoho, e leng se ka bakang liphetoho ts'ebetsong ea liphatsa tsa lefutso. Ho latela lithuto tse seng li ntse li le teng, re boetse re fumane hore palo ea li-intron e ne e amahanngoa le tlhahiso ea liphatsa tsa lefutso. Ha palo ea li-introns liphatseng tsa lefutso e le kholo, liphatsa tsa lefutso li ka arabela kapele linthong tse fapaneng tse sa thabiseng.
Ho pheta-pheta ha liphatsa tsa lefutso ke ntlha e kholo phetohong ea liphatsa tsa lefutso le liphatsa tsa lefutso37. Lithuto tse amanang li bontšitse hore ho pheta-pheta ha liphatsa tsa lefutso ha ho eketse feela palo ea liphatsa tsa lefutso tsa GRF, empa hape ho sebetsa e le mokhoa oa ho hlahisa liphatsa tsa lefutso tse ncha ho thusa limela ho ikamahanya le maemo a fapaneng a mabe a tikoloho38. Palo eohle ea lipara tsa liphatsa tsa lefutso tse 48 tse tšoanang li fumanoe thutong ena, tseo kaofela e neng e le ho pheta-pheta ha likarolo, ho bontšang hore ho pheta-pheta ha likarolo ke mokhoa o moholo oa ho eketsa palo ea liphatsa tsa lefutso lelapeng lena. Ho tlalehiloe libukeng hore ho pheta-pheta ha likarolo ho ka khothaletsa ka katleho ho holisoa ha litho tsa lelapa la liphatsa tsa lefutso tsa GRF ho Arabidopsis le fragola, 'me ha ho na ho pheta-pheta ha sehlopha sena sa liphatsa tsa lefutso ho fumanoeng ho mofuta ofe kapa ofe oa 27,39. Liphetho tsa thuto ena li lumellana le lithuto tse seng li ntse li le teng mabapi le malapa a Arabidopsis thaliana le fragola, ho fana ka maikutlo a hore lelapa la GRF le ka eketsa palo ea liphatsa tsa lefutso le ho hlahisa liphatsa tsa lefutso tse ncha ka ho pheta-pheta ha likarolo limela tse fapaneng.
Phuputsong ena, liphatsa tsa lefutso tse 34 tsa BjGRF li ile tsa fumanoa mosetareteng, tse ileng tsa aroloa ka malapa a manyenyane a 3. Liphatsa tsena tsa lefutso li bontšitse mekhoa e tšoanang e bolokiloeng le libopeho tsa liphatsa tsa lefutso. Tlhahlobo ea Collinearity e senotse lipara tse 48 tsa ho pheta-pheta ha likarolo mosetareteng. Sebaka sa promoter sa BjGRF se na le likarolo tse sebetsang tsa cis tse amanang le karabelo ea leseli, karabelo ea lihormone, karabelo ea khatello ea tikoloho, le kholo le nts'etsopele. Pontšo ea liphatsa tsa lefutso tse 34 tsa BjGRF e fumanoe mohatong oa peō ea mosetareta (metso, likutu, makhasi), le mokhoa oa tlhahiso ea liphatsa tsa lefutso tse 10 tsa BjGRF tlas'a maemo a komello. Ho fumanoe hore mekhoa ea tlhahiso ea liphatsa tsa lefutso tsa BjGRF tlas'a khatello ea komello e ne e tšoana 'me e ka tšoana. ho kenella komellong Ho qobella taolo. Liphatsa tsa lefutso tsa BjGRF03 le BjGRF32 li ka bapala karolo e ntle ea taolo khatellong ea komello, ha BjGRF06 le BjGRF23 li bapala karolo khatellong ea komello e le liphatsa tsa lefutso tse reretsoeng miR396. Ka kakaretso, thuto ea rona e fana ka motheo oa baeloji bakeng sa ho sibolloa ha mosebetsi oa liphatsa tsa lefutso tsa BjGRF nakong e tlang limela tsa Brassicaceae.
Peo ea mosetareta e sebelisitsoeng tekong ena e fanoe ke Setsi sa Lipatlisiso tsa Peo ea Oli sa Guizhou, Sekolo sa Mahlale a Temo sa Guizhou. Khetha peo eohle 'me u e jale mobung (substrate: mobu = 3:1), 'me u bokelle metso, likutu le makhasi kamora mohato oa makhasi a mane. Limela li ile tsa phekoloa ka 20% PEG 6000 ho etsisa komello, 'me makhasi a ile a bokelloa kamora lihora tse 0, 3, 6, 12 le 24. Lisampole tsohle tsa limela li ile tsa hoamisoa hang-hang ka naetrojene e metsi ebe li bolokoa ka sehatsetsing sa -80°C bakeng sa teko e latelang.
Lintlha tsohle tse fumanoeng kapa tse hlahlobiloeng nakong ea phuputso ena li kenyelelitsoe sehloohong se phatlalalitsoeng le lifaeleng tsa tlhahisoleseling e eketsehileng.
Nako ea poso: Pherekhong-22-2025