Kabo ea sehla ea lipula Profinseng ea Guizhou ha e lekane, ho na le lipula tse ngata nakong ea selemo le lehlabula, empa lipeo tsa peō ea rapese li kotsing ea khatello ea komello nakong ea hoetla le mariha, e amang chai haholo. Mustard ke sejalo se khethehileng sa peo ea oli haholo-holo se lengoang Profinseng ea Guizhou. E na le mamello e matla ea komello 'me e ka lengoa libakeng tse lithaba. Ke mohloli o ruileng oa liphatsa tsa lefutso tse hanyetsanang le komello. Ho sibolloa ha liphatsa tsa lefutso tse hanyetsanang le komello ho bohlokoa haholo bakeng sa ntlafatso ea mefuta ea mosetareta. le popontshwa ho mehloli ya germplasm. Lelapa la GRF le phetha karolo ea bohlokoa kholong ea limela le nts'etsopele le karabelo khatellong ea komello. Hajoale, liphatsa tsa lefutso tsa GRF li fumanoe ho Arabidopsis 2, raese (Oryza sativa) 12, rapeseed 13, cotton (Gossypium hirsutum) 14, koro (Triticum). aestivum)15, pearl millet (Setaria italica)16 le Brassica17, empa ha ho na litlaleho tsa liphatsa tsa lefutso tsa GRF tse fumanoeng mosetareteng. Thutong ena, liphatsa tsa lefutso tsa lelapa la GRF tsa mosetareta li ile tsa khetholloa boemong ba genome-wide le litšobotsi tsa bona tsa 'mele le lik'hemik'hale, likamano tsa ho iphetola ha lintho, homology, li-conserved motifs, sebopeho sa liphatsa tsa lefutso, liphatsa tsa lefutso, li-cis-elements le sethaleng sa sethopo (sekhahla sa makhasi a mane) se ile sa hlahlojoa. Lipolelo tse tlas'a khatello ea komello li ile tsa hlahlobjoa ka botlalo ho fana ka motheo oa saense bakeng sa lithuto tse tsoelang pele mabapi le ts'ebetso e ka bang teng ea liphatsa tsa lefutso tsa BjGRF karabelong ea komello le ho fana ka liphatsa tsa lefutso bakeng sa ho ikatisa mosetareta o mamellang komello.
Liphatsa tsa lefutso tse mashome a mararo a metso e mene tsa BjGRF li ile tsa khetholloa ho Brassica juncea genome ho sebelisoa lipatlisiso tse peli tsa HMMER, tseo kaofela li nang le libaka tsa QLQ le WRC. Tatelano ea CDS ea liphatsa tsa lefutso tsa BjGRF tse khethiloeng li hlahisoa ho Supplementary Table S1. BjGRF01–BjGRF34 li rehiloe ho latela sebaka sa tsona ho chromosome. Lintho tsa physicochemical tsa lelapa lena li bontša hore bolelele ba amino acid bo fapane haholo, ho tloha ho 261 aa (BjGRF19) ho ea ho 905 aa (BjGRF28). Sebaka sa isoelectric sa BjGRF se tloha ho 6.19 (BjGRF02) ho ea 9.35 (BjGRF03) ka karolelano ea 8.33, le 88.24% ea BjGRF ke protheine ea motheo. Boima ba limolek'hule bo boletsoeng esale pele ba BjGRF bo tsoa ho 29.82 kDa (BjGRF19) ho ea ho 102.90 kDa (BjGRF28); index ea ho se tsitse ha liprotheine tsa BjGRF ho tloha ho 51.13 (BjGRF08) ho ea ho 78.24 (BjGRF19), kaofela li kholo ho feta 40, e leng se bontšang hore index ea mafura a mafura e tloha ho 43.65 (BjGRF01) ho ea ho 78.78 (BjGRF22), karolelano ea 78.78 (BjGRF22) hydro. (BjGRF31) ho ea ho -0.45 (BjGRF22), liprotheine tsohle tsa hydrophilic BjGRF li na le litekanyetso tse mpe tsa GRAVY, tse ka 'nang tsa bakoa ke khaello ea hydrophobicity e bakoang ke masala. Polelo ea sebaka sa sebaka sa subcellular e bonts'itse hore liprotheine tse kentsoeng tsa 31 BjGRF li ka beoa sebakeng sa mantlha, BjGRF04 e ka beoa sebakeng sa peroxisomes, BjGRF25 e ka beoa sebakeng sa cytoplasm, mme BjGRF28 e ka beoa sebakeng sa li-chloroplasts (Letlapa la 1), e bonts'a hore karolo ea bohlokoa ea BjGRF e bonts'a hore karolo ea bohlokoa ea BjGRF ntlha ea mongolo.
Tlhahlobo ea phylogenetic ea malapa a GRF ka mefuta e fapaneng e ka thusa ho ithuta mesebetsi ea liphatsa tsa lefutso. Ka hona, tatelano ea amino acid ea bolelele bo felletseng ea 35 rapeseed, 16 turnip, 12 raese, millet e 10 le 9 Arabidopsis GRFs e ile ea jarolloa 'me sefate sa phylogenetic se hahiloe ho ipapisitsoe le liphatsa tsa lefutso tse 34 tsa BjGRF (setšoan. 1). Malapa a mararo a na le palo e fapaneng ea litho; 116 GRF TF e arotsoe ka malapa a mararo a fapaneng (lihlopha A~C), tse nang le 59 (50.86%), 34 (29.31%) le 23 (19.83)% ea li-GRF, ka ho latellana. Har'a bona, litho tse 34 tsa lelapa la BjGRF li hasane ka har'a malapa a 3: litho tse 13 sehlopheng sa A (38.24%), litho tse 12 sehlopheng sa B (35.29%) le litho tse 9 sehlopheng sa C (26.47%). Ts'ebetsong ea polyploidization ea mosetareta, palo ea liphatsa tsa lefutso tsa BjGRF ka malapa a fapaneng a fapane, 'me ho ka etsahala hore liphatsa tsa lefutso le tahlehelo li hlahe. Ho bohlokoa ho hlokomela hore ha ho na kabo ea li-GRF tsa raese le nyalothe sehlopheng sa C, ha ho na le li-GRF tse 2 tsa raese le nyalothe e le 'ngoe ea GRF sehlopheng sa B, 'me boholo ba li-GRF tsa raese le nyalothe li arotsoe ka lekala le le leng, ho bontša hore BjGRFs e amana haufi-ufi le dicots. Har'a tsona, liphuputso tse tebileng ka ho fetisisa tsa mosebetsi oa GRF ho Arabidopsis thaliana li fana ka motheo oa lithuto tse sebetsang tsa BjGRF.
Sefate sa Phylogenetic sa mosetareta se kenyeletsang Brassica napus, Brassica napus, raese, nyalothe le litho tsa lelapa la Arabidopsis thaliana GRF.
Tlhahlobo ea liphatsa tsa lefutso tse pheta-phetoang lelapeng la mustard GRF. Mola o moputsoa o ka morao o emela boloko bo lumellanang ka har'a genome ea mosetareta, mola o mofubelu o emela likarolo tse peli tse pheta-phetoang tsa mofuta oa BjGRF;
BjGRF ea liphatsa tsa lefutso tlas'a khatello ea komello sethaleng sa bone sa makhasi. Lintlha tsa qRT-PCR li bonts'oa ho Supplementary Table S5. Phapano e kholo ea data e bonts'oa ka litlhaku tse nyane.
Ha boemo ba leholimo ba lefats'e bo ntse bo tsoela pele ho fetoha, ho ithuta ka moo lijalo li sebetsanang le khatello ea komello le ho ntlafatsa mekhoa ea tsona ea mamello e fetohile sehlooho se chesang sa lipatlisiso18. Ka mor'a komello, sebopeho sa morphological, liphatsa tsa lefutso le mekhoa ea metsoako ea limela e tla fetoha, e leng se ka lebisang ho emisoa ha photosynthesis le tšitiso ea metabolism, e amang chai le boleng ba lijalo19,20,21. Ha limela li utloa matšoao a komello, li hlahisa manģosa a bobeli a kang Ca2 + le phosphatidylinositol, ho eketsa intracellular calcium ion concentration le ho kenya tšebetsong marang-rang a taolo ea protheine phosphorylation pathway22,23. Protheine ea ho qetela ea sepheo e ameha ka ho toba ts'ireletso ea lisele kapa e laola polelo ea liphatsa tsa lefutso tse amanang le khatello ea kelello ka li-TF, ho matlafatsa mamello ea limela ho khatello ea maikutlo24,25. Kahoo, li-TF li phetha karolo ea bohlokoa ho arabeleng khatello ea komello. Ho ea ka tatellano le thepa e tlamang ea DNA ea li-TF tse arabelang khatello ea komello, li-TF li ka aroloa ka malapa a fapaneng, joalo ka GRF, ERF, MYB, WRKY le malapa a mang26.
Lelapa la liphatsa tsa lefutso tsa GRF ke mofuta oa TF e tobileng semela e bapalang likarolo tsa bohlokoa likarolong tse fapaneng tse kang kholo, nts'etsopele, phetisetso ea matšoao le likarabo tsa tšireletso ea semela27. Ho tloha ha mofuta oa pele oa GRF o khetholloa ho O. sativa28, liphatsa tsa lefutso tse ngata tsa GRF li 'nile tsa khetholloa mefuteng e mengata' me li bontšoa ho ama kholo ea limela, tsoelo-pele le karabelo ea khatello ea maikutlo8, 29, 30,31,32. Ka phatlalatso ea tatellano ea liphatsa tsa lefutso tsa Brassica juncea, ho tsebahatsoa ha leloko la lefutso la BjGRF ho ile ha khoneha33. Phuputsong ena, liphatsa tsa lefutso tse 34 tsa BjGRF li ile tsa khetholloa ka har'a genome eohle ea mosetareta 'me tsa bitsoa BjGRF01-BjGRF34 ho latela boemo ba tsona ba chromosomal. Kaofela ha tsona li na le libaka tse sirelelitsoeng haholo tsa QLQ le WRC. Tlhahlobo ea thepa ea physicochemicals e bontšitse hore phapang ea linomoro tsa amino acid le boima ba limolek'hule tsa liprotheine tsa BjGRF (ntle le BjGRF28) e ne e se ea bohlokoa, e bontšang hore litho tsa lelapa la BjGRF li ka ba le mesebetsi e tšoanang. Tlhahlobo ea sebopeho sa liphatsa tsa lefutso e bontšitse hore 64.7% ea liphatsa tsa lefutso tsa BjGRF li na le li-exons tse 4, e leng se bontšang hore sebopeho sa liphatsa tsa lefutso tsa BjGRF se batla se bolokiloe ka ho iphetola ha lintho, empa palo ea li-exons ho liphatsa tsa lefutso tsa BjGRF10, BjGRF16, BjGRP28 le BjGRF29 e kholoanyane. Liphuputso li bontšitse hore ho phaella kapa ho tlosoa ha li-exons kapa li-introns ho ka lebisa ho se tšoane ha sebopeho sa liphatsa tsa lefutso le mosebetsi, kahoo ho theha mefuta e mecha34,35,36. Ka hona, re nahana hore intron ea BjGRF e ile ea lahleha nakong ea ho iphetola ha lintho, e ka bakang liphetoho mosebetsing oa liphatsa tsa lefutso. Tumellanong le lithuto tse teng, re fumane hape hore palo ea li-intron e ne e amahanngoa le polelo ea liphatsa tsa lefutso. Ha palo ea li-intron ho liphatsa tsa lefutso e le khōlō, liphatsa tsa lefutso li ka arabela ka potlako linthong tse sa tšoaneng tse sa thabiseng.
Ho pheta-pheta liphatsa tsa lefutso ke ntlha e kholo ea ho fetoha ha liphatsa tsa lefutso le liphatsa tsa lefutso37. Liphuputso tse amanang le tsona li bontšitse hore phetiso ea liphatsa tsa lefutso ha e eke feela palo ea liphatsa tsa lefutso tsa GRF, empa hape e sebetsa e le mokhoa oa ho hlahisa liphatsa tsa lefutso tse ncha ho thusa limela ho ikamahanya le maemo a fapaneng a mabe a tikoloho38. Kakaretso ea liphatsa tsa lefutso tse 48 tse kopilitsoeng li fumanoe thutong ena, tseo kaofela e neng e le likopi tsa likaroloana, tse bontšang hore ho ikatisa ka likarolo ke mokhoa o ka sehloohong oa ho eketsa palo ea liphatsa tsa lefutso lelapeng lena. Ho 'nile ha tlalehoa libukeng hore ho pheta-pheta ka lihlopha ho ka khothalletsa ka katleho ho holisa litho tsa lelapa la GRF liphatsa tsa lefutso ho Arabidopsis le fragola,' me ha ho phetiso ea tandem ea mofuta ona oa lefutso e fumanoeng ho mofuta ofe kapa ofe oa mefuta27,39. Liphetho tsa phuputso ena li lumellana le liphuputso tse seng li ntse li le teng ka malapa a Arabidopsis thaliana le fragola, tse fanang ka maikutlo a hore lelapa la GRF le ka eketsa palo ea liphatsa tsa lefutso le ho hlahisa liphatsa tsa lefutso tse ncha ka ho pheta-pheta ka likarolo limeleng tse fapaneng.
Phuputsong ena, kakaretso ea liphatsa tsa lefutso tse 34 tsa BjGRF li ile tsa khetholloa ka mosetareta, tse arotsoeng ka malapa a 3. Liphatsa tsa lefutso tsena li ne li bontša mekhoa e ts'oanang e bolokiloeng le liphatsa tsa lefutso. Tlhahlobo ea Collinearity e senotse lipara tse 48 tsa likarolo tse kopitsoang ka mosetareta. Sebaka sa papatso sa BjGRF se na le likarolo tsa cis-acting tse amanang le karabelo e bobebe, karabelo ea lihormone, karabelo ea khatello ea tikoloho, kholo le nts'etsopele. Polelo ea liphatsa tsa lefutso tsa 34 BjGRF e ile ea fumanoa sethaleng sa sethopo sa mosetareta (metso, stems, makhasi), le mokhoa oa ho hlahisa liphatsa tsa lefutso tsa 10 BjGRF tlas'a maemo a komello. Ho ile ha fumanoa hore mekhoa ea polelo ea liphatsa tsa lefutso tsa BjGRF tlas'a khatello ea komello e ne e tšoana 'me e ka tšoana. ho kenya letsoho komelong Ho qobella taolo. Liphatsa tsa lefutso tsa BjGRF03 le BjGRF32 li ka ba le likarolo tse ntle tsa taolo khatellong ea komello, ha BjGRF06 le BjGRF23 li bapala karolo khatellong ea komello joalo ka liphatsa tsa lefutso tsa miR396. Ka kakaretso, thuto ea rona e fana ka motheo oa likokoana-hloko bakeng sa ho sibolloa ha nako e tlang ea BjGRF ea liphatsa tsa lefutso ho limela tsa Brassicaceae.
Peō ea mosetareta e sebelisitsoeng tekong ena e fanoe ke Guizhou Oil Seed Research Institute, Guizhou Academy of Agricultural Sciences. Kgetha dipeo kaofela mme o di leme mobung (substrate: mobu = 3:1), mme o bokelle metso, dikutu le makgasi ka mora mokgahlelo wa makgasi a mane. Limela li ile tsa phekoloa ka 20% PEG 6000 ho etsisa komello, 'me makhasi a bokelloa ka mor'a lihora tse 0, 3, 6, 12 le 24. Mehlala eohle ea limela e ile ea hoamisoa hang-hang ka har'a metsi a naetrojene 'me ea bolokoa ka sehatsetsing sa -80°C bakeng sa teko e latelang.
Lintlha tsohle tse fumanoeng kapa tse hlahlobiloeng nakong ea phuputso ena li kenyelelitsoe sehloohong se hatisitsoeng le lifaele tsa boitsebiso bo eketsehileng.
Nako ea poso: Jan-22-2025