Li-mushroom tse kholo li na le sete e ruileng le e fapaneng ea li-metabolite tse sebetsang 'meleng' me li nkoa e le mehloli ea bohlokoa ea bioresource. Phellinus igniarius ke li-mushroom tse kholo tse sebelisoang ka tloaelo bakeng sa merero ea bongaka le ea lijo, empa sehlopha sa tsona le lebitso la Selatine li ntse li phehisana khang. Ka ho sebelisa tlhahlobo ea ho hokahanya likarolo tsa liphatsa tsa lefutso tse ngata, bafuputsi ba tiisitse hore Phellinus igniarius le mefuta e tšoanang ke ea mofuta o mocha 'me ba thehile mofuta oa Sanghuangporus. Li-mushroom tsa honeysuckle Sanghuangporus lonicericola ke e 'ngoe ea mefuta ea Sanghuangporus e tsebahalang lefatšeng ka bophara. Phellinus igniarius e hohetse tlhokomelo e kholo ka lebaka la thepa ea eona e fapaneng ea bongaka, ho kenyeletsoa li-polysaccharides, li-polyphenols, li-terpenes le li-flavonoid. Li-Triterpenes ke metsoako ea bohlokoa e sebetsang ka meriana ea mofuta ona, e bonts'ang mesebetsi ea antioxidant, antibacterial le antitumor.
Li-triterpenoid li na le bokhoni bo boholo ba ho sebelisoa khoebong. Ka lebaka la ho se fumanehe ha mehloli ea tlhaho ea Sanghuangporus, ho ntlafatsa katleho ea eona ea biosynthetic le chai ke habohlokoa haholo. Hona joale, ho entsoe tsoelo-pele ho ntlafatseng tlhahiso ea li-metabolite tse fapaneng tsa bobeli tsa Sanghuangporus ka ho sebelisa lik'hemik'hale tse susumetsang ho laola maano a ho belisoa ka metsing. Mohlala, li-acid tse mafura tse nang le polyunsaturated, li-ecitors tsa fungal11 le li-phytohormones (ho kenyeletsoa le methyl jasmonate le salicylic acid14) li bontšitsoe li eketsa tlhahiso ea triterpenoid ho Sanghuangporus. Balaoli ba kholo ea limela(Li-PGR)e ka laola biosynthesis ea metabolites ea bobeli limela. Phuputsong ena, PBZ, molaoli oa kholo ea limela o sebelisoang haholo ho laola kholo ea limela, chai, boleng le litšobotsi tsa 'mele, o ile oa hlahlojoa. Haholo-holo, tšebeliso ea PBZ e ka susumetsa tsela ea biosynthetic ea terpenoid limela. Motsoako oa gibberellins le PBZ o ekelitse litaba tsa quinone methide triterpene (QT) ho Montevidia floribunda. Sebopeho sa tsela ea terpenoid ea oli ea lavender se fetotsoe kamora kalafo ka 400 ppm PBZ. Leha ho le joalo, ha ho na litlaleho mabapi le ts'ebeliso ea PBZ ho li-mushroom.
Ntle le lithuto tse shebaneng le keketseho ea tlhahiso ea triterpene, lithuto tse ling li boetse li hlakisitse mekhoa ea taolo ea triterpene biosynthesis ho Moriformis tlas'a tšusumetso ea lik'hemik'hale tse susumetsang. Hona joale, lithuto li shebane le phetoho ea maemo a tlhahiso ea liphatsa tsa lefutso tsa sebopeho tse amanang le triterpene biosynthesis tseleng ea MVA, e leng se lebisang keketsehong ea tlhahiso ea terpenoid.12,14 Leha ho le joalo, litsela tse ka tlas'a liphatsa tsena tse tsejoang tsa sebopeho, haholo-holo lintlha tsa phetolelo tse laolang tlhahiso ea tsona, li ntse li sa hlaka mekhoeng ea taolo ea triterpene biosynthesis ho Moriformis.
Phuputsong ena, ho ile ha fuputswa ditlamorao tsa maemo a fapaneng a balaodi ba kgolo ya dimela (PGRs) tlhahisong ya triterpene le kgolo ya mycelial nakong ya ho belisoa ha honeysuckle (S. lonicericola) ka tlasa metsi. Ka mora moo, metabolomics le transcriptomics di ile tsa sebediswa ho sekaseka sebopeho sa triterpene le dipaterone tsa tlhahiso ya dijini tse amehang ho etseng dijini tsa triterpene nakong ya kalafo ya PBZ. Dintlha tsa tatellano ya RNA le bioinformatics di ile tsa hlwaya hape ntlha ya ho ngolwa ha target ya MYB (SlMYB). Ho feta moo, diphetoho di ile tsa hlahiswa ho netefatsa phello ya taolo ya jini ya SlMYB hodima etseng dijini tsa triterpene le ho hlwaya dijini tse ka bang teng. Diteko tsa electrophoretic mobility shift assays (EMSA) di ile tsa sebediswa ho netefatsa tshebedisano ya protheine ya SlMYB le bahlohlelletsi ba jini ya target ya SlMYB. Ka bokhutšoanyane, sepheo sa phuputso ena e ne e le ho hlasimolla triterpene biosynthesis ho sebelisoa PBZ le ho khetholla MYB transcription factor (SlMYB) e laolang ka ho toba liphatsa tsa lefutso tsa triterpene biosynthesis ho kenyeletsoa MVD, IDI, le FDPS ho S. lonicericola ho arabela tlhahiso ea PBZ.
Ho kenngoa ha IAA le PBZ ho ekelitse tlhahiso ea triterpenoid haholo ho honeysuckle, empa phello ea ho kenngoa ha PBZ e ne e totobetse haholoanyane. Ka hona, PBZ e fumanoe e le ntho e ntlafatsang ka ho fetisisa ka khatello e eketsehileng ea 100 mg/L, e lokelang ho ithutoa haholoanyane.
Nako ea poso: Phato-19-2025