Liprotheine tsa DELLA lia bolokoabalaoli ba kholotse bapalang karolo ea bohlokoa nts'etsopele ea limela ho arabela matšoao a ka hare le a kantle. Jwalo ka balaoli ba phetolelo, DELLA e tlama ho lintlha tsa phetolelo (TFs) le histone H2A ka libaka tsa bona tsa GRAS mme e hiriloe ho sebetsa ho batšehetsi. Lithuto tsa morao tjena li bontšitse hore botsitso ba DELLA bo laoloa kamora phetolelo ka mekhoa e 'meli: polyubiquitination e bakoang ke hormone ea semela.gibberellin, e leng se lebisang ho senyeha ha tsona ka potlako, le ho kopanngoa le modifier e nyane e kang ubiquitin (SUMO), e eketsang pokello ea tsona. Ho feta moo, ts'ebetso ea DELLA e laoloa ka matla ke mekhoa e 'meli e fapaneng ea glycosylation: O-fucosylation e ntlafatsa tšebelisano ea DELLA-TF, athe phetoho ea O-linked N-acetylglucosamine (O-GlcNAc) e thibela tšebelisano ea DELLA-TF. Leha ho le joalo, karolo ea phosphorylation ea DELLA ha e hlake kaha lithuto tsa pejana li bontšitse liphetho tse hanyetsanang, tse ling li fana ka maikutlo a hore phosphorylation e khothalletsa kapa e hatella ho senyeha ha DELLA 'me tse ling li fana ka maikutlo a hore phosphorylation ha e ame botsitso ba tsona. Mona, re khetholla libaka tsa phosphorylation ho GA1-3 repressor (RGA), AtDELLA, e hloekisitsoeng ho Arabidopsis thaliana ka mass spectrometry 'me re bontša hore phosphorylation ea li-peptide tse peli tsa RGA libakeng tsa PolyS le PolyS/T e ntlafatsa ts'ebetso ea RGA ka ho khothaletsa ho tlamahana ha H2A le kamano ea RGA le batšehetsi ba shebiloeng. Haholo-holo, phosphorylation ha ea ka ea ama likamano tsa RGA-TF kapa botsitso ba RGA. Phuputso ea rona e senola mokhoa oa limolek'hule oo phosphorylation e susumetsang ts'ebetso ea DELLA ka oona.
Tlhahlobo ea rona ea mass spectrometric e senotse hore Pep1 le Pep2 li ne li na le phosphorylation e ngata ho RGA ka lebaka la Ga1 e haellang GA. Ntle le phuputso ena, lithuto tsa phosphoproteomic le tsona li senotse phosphorylation ea Pep1 ho RGA, leha karolo ea eona e so ithutiloe53,54,55. Ka lehlakoreng le leng, phosphorylation ea Pep2 ha e so hlalosoe pele kaha peptide ena e ne e ka fumanoa feela ka ho sebelisa transgene ea RGAGKG. Le hoja phetoho ea m1A, e ileng ea felisa phosphorylation ea Pep1, e fokolitse mosebetsi oa RGA hanyane feela ho limela, e bile le phello ea tlatsetso ha e kopantsoe le m2A ho fokotsa mosebetsi oa RGA (Setšoantšo se Tlatsetsang sa 6). Habohlokoa, phosphorylation ea Pep1 e fokotsehile haholo ho sly1 mutant e ntlafalitsoeng ke GA ha e bapisoa le ga1, e leng se bontšang hore GA e khothalletsa dephosphorylation ea RGA, e fokotsa mosebetsi oa eona. Mokhoa oo GA e hatellang phosphorylation ea RGA ka oona o hloka lipatlisiso tse ling. Monyetla o mong ke hore sena se finyelloa ka taolo ea protheine kinase e sa tsejoeng. Leha diphuputso di bontshitse hore tlhahiso ya protheine ya CK1 kinase EL1 e fokotswa ke GA ka hara raese41, diphetho tsa rona di bontsha hore diphetoho tse phahameng tsa Arabidopsis EL1 homologue (AEL1-4) ha di fokotse phosphorylation ya RGA. Tumellanong le diphetho tsa rona, phuputso ya moraorao ya phosphoproteomic e sebedisang mela e feteletseng ya Arabidopsis AEL le ael triple mutant ha e a ka ya hlwaya diprotheine tsa DELLA e le di-substrate tsa kinases tsena56. Ha re lokisa sengolwa, ho ile ha tlalehwa hore GSK3, jini e kenyang kinase e kang GSK3/SHAGGY korong (Triticum aestivum), e ka fosforisa DELLA (Rht-B1b)57, le hoja phosphorylation ya Rht-B1b ka GSK3 e so tiisetswe dimeleng. Likarabelo tsa enzymatic tsa in vitro boteng ba GSK3 tse lateloang ke tlhahlobo ea mass spectrometry li senotse libaka tse tharo tsa phosphorylation tse pakeng tsa libaka tsa DELLA le GRAS tsa Rht-B1b (Setšoantšo se Tlatsetsang sa 3). Ho nkeloa sebaka ha serine ho ea ho alanine libakeng tsohle tse tharo tsa phosphorylation ho bakile ho fokotseha ha ts'ebetso ea Rht-B1b korong ea transgenic, ho latela liphuputso tsa rona tsa hore ho nkeloa sebaka ha alanine ho Pep2 RGA ho fokolitse ts'ebetso ea RGA. Leha ho le joalo, liteko tsa ho senyeha ha protheine ea in vitro li bontšitse hape hore phosphorylation e ka boela ea tsitsisa Rht-B1b57. Sena se fapane le liphetho tsa rona tse bontšang hore ho nkeloa sebaka ha alanine ho Pep2 RGA ha ho fetole botsitso ba eona limela. GSK3 korong ke ortholog ea protheine e sa utloeng bohloko ea brassinosteroid 2 (BIN2) ho Arabidopsis 57. BIN2 ke taolo e mpe ea lets'oao la BR, 'me BR e kenya tšebetsong tsela ea eona ea lets'oao ka ho baka ho senyeha ha BIN2 58. Re bontšitse hore kalafo ea BR ha e fokotse botsitso ba RGA 59 kapa maemo a phosphorylation ho Arabidopsis (Setšoantšo se Tlatsetsang sa 2), e leng se bontšang hore RGA ha e khone ho phosphorylation ke BIN2.
Lintlha tsohle tsa bongata li hlahlobiloe ka lipalo-palo ho sebelisoa Excel, 'me liphapang tse kholo li ile tsa fumanoa ho sebelisoa teko ea t ea Seithuti. Ha ho na mekhoa ea lipalo-palo e sebelisitsoeng ho fumana boholo ba sampole esale pele. Ha ho na data e ileng ea tlosoa tlhahlobong; teko ha ea ka ea etsoa ka mokhoa o sa reroang; 'me bafuputsi ba ne ba tseba kabo nakong ea teko le tlhahlobo ea sephetho. Boholo ba lisampole bo fanoa litšōmong tsa lipalo le lifaeleng tsa data tse tala.
Nako ea poso: Mmesa-15-2025