Liprotheine tsa DELLA lia baballoabalaoli ba kholotse phethang karolo e ka sehloohong ntshetsopeleng ya dimela karabelong ya matshwao a ka hare le kantle. Joaloka balaoli ba mongolo, li-DELLA li tlamella lintlha tse ngotsoeng (TFs) le histone H2A ka libaka tsa tsona tsa GRAS 'me li thaothoa ho sebetsa ho batšehetsi. Liphuputso tsa morao-rao li bontšitse hore botsitso ba DELLA bo laoloa ka mor'a phetolelo ka mekhoa e 'meli: polyubiquitination e bakoang ke hormone ea semela.gibberellin, e leng se lebisang ho senyeha ha bona ka potlako, le ho kopanya ka mokhoa o monyenyane oa ubiquitin-like modifier (SUMO), e leng ho eketsang ho bokella ha bona. Ho feta moo, ts'ebetso ea DELLA e laoloa ka matla ke mekhoa e 'meli e fapaneng ea glycosylation: O-fucosylation e ntlafatsa tšebelisano ea DELLA-TF, athe phetoho ea O-linked N-acetylglucosamine (O-GlcNAc) e thibela tšebelisano ea DELLA-TF. Leha ho le joalo, karolo ea DELLA phosphorylation ha e hlake kaha liphuputso tse fetileng li bontšitse liphello tse hanyetsanang, ba bang ba fana ka maikutlo a hore phosphorylation e khothalletsa kapa e hatella ho senyeha ha DELLA 'me ba bang ba fana ka maikutlo a hore phosphorylation ha e ame botsitso ba bona. Mona, re khetholla libaka tsa phosphorylation ho GA1-3 repressor (RGA), AtDELLA, e hloekisitsoeng ho tloha Arabidopsis thaliana ka mass spectrometry le ho bontša hore phosphorylation ea li-peptide tse peli tsa RGA libakeng tsa PolyS le PolyS / T li ntlafatsa mosebetsi oa RGA ka ho khothalletsa ho tlama ha H2A le ho kopana le RGA le bahlahisi ba sepheo. Haholo-holo, phosphorylation ha ea ka ea ama litšebelisano tsa RGA-TF kapa botsitso ba RGA. Boithuto ba rona bo senola mokhoa oa limolek'hule oo phosphorylation e susumetsang ts'ebetso ea DELLA.
Tlhahlobo ea rona ea "masight spectrometric" e senoletse hore Pep1 le Pep2 li ne li le phosphorylated haholo ho RGA mokokotlong oa Ga1 o haelloang ke GA. Ntle le thuto ena, lithuto tsa phosphoproteomic li boetse li senotse Pep1 phosphorylation ho RGA, le hoja karolo ea eona e e-s'o ithutoe53,54,55. Ka lehlakoreng le leng, phosphorylation ea Pep2 ha e e-s'o hlalosoe pele kaha peptide ena e ne e ka fumanoa feela ho sebelisa RGAGKG transgene. Leha phetoho ea m1A, e felisitseng phosphorylation ea Pep1, e fokolitse ts'ebetso ea RGA hanyenyane feela ho semela, e bile le phello ea tlatsetso ha e kopantsoe le m2A ho fokotsa ts'ebetso ea RGA (Figure ea Tlatsetso 6). Habohlokoa, Pep1 phosphorylation e fokotsehile haholo ho GA-enhanced sly1 mutant ha e bapisoa le ga1, e fana ka maikutlo a hore GA e khothalletsa RGA dephosphorylation, e fokotsa mosebetsi oa eona. Mochine oo GA e hatellang RGA phosphorylation o hloka lipatlisiso tse eketsehileng. Monyetla o mong ke hore sena se finyelloa ka ho laola protheine kinase e sa tsejoeng. Leha liphuputso li bonts'itse hore polelo ea CK1 protein kinase EL1 e theotsoe ke GA ka rice41, liphetho tsa rona li bonts'a hore liphetoho tse phahameng tsa Arabidopsis EL1 homologue (AEL1-4) ha li fokotse RGA phosphorylation. Tumellanong le liphetho tsa rona, thuto ea morao-rao ea phosphoproteomic e sebelisang melapo ea Arabidopsis AEL overexpressing le ael triple mutant ha ea ka ea khetholla liprotheine tsa DELLA e le li-substrates tsa kinases56. Ha re lokisetsa buka e ngotsoeng ka letsoho, ho ile ha tlalehoa hore GSK3, gene e kenyang GSK3 / SHAGGY-joaloka kinase ka koro (Triticum aestivum), e ka phosphorylate DELLA (Rht-B1b)57, le hoja phosphorylation ea Rht-B1b ke GSK3 e sa netefatsoa semela. In vitro enzymatic reactions ka pel'a GSK3 e lateloa ke tlhahlobo ea spectrometry ea boima e senotse libaka tse tharo tsa phosphorylation tse pakeng tsa libaka tsa DELLA le GRAS tsa Rht-B1b (Supplementary Fig. 3). Li-substitutes tsa Serine ho alanine libakeng tsohle tse tharo tsa phosphorylation li entse hore mosebetsi oa Rht-B1b o fokotsehe ka koro ea transgenic, ho latela liphuputso tsa rona tsa hore li-alanine substitutes ho Pep2 RGA li fokolitse mosebetsi oa RGA. Leha ho le joalo, liteko tsa ho senyeha ha protheine ea in vitro li bontšitse hape hore phosphorylation e ka boela ea tsitsisa Rht-B1b57. Sena se fapane le liphetho tsa rona tse bonts'ang hore li-substitutes tsa alanine ho Pep2 RGA ha li fetole botsitso ba eona ho semela. GSK3 ka koro ke ortholog ea brassinosteroid-insensitive protein 2 (BIN2) ho Arabidopsis 57. BIN2 ke molaoli o mobe oa pontšo ea BR, 'me BR e kenya tšebetsong tsela ea eona ea pontšo ka ho etsa hore BIN2 e senyehe 58. Re bontšitse hore phekolo ea BR ha e fokotse RGA botsitso ba 59 kapa phosphory2 ea phosphory (phosphory) ea RGA ea phosphory (phosphory) e fana ka maikutlo a hore RGA ha e na monyetla oa ho ba phosphorylated ke BIN2.
Lintlha tsohle tsa palo li ile tsa hlahlobjoa ka lipalo ho sebelisoa Excel, 'me liphapang tse kholo li ile tsa khethoa ho sebelisoa tlhahlobo ea t ea Seithuti. Ha ho mekhoa ea lipalo-palo e sebelisitsoeng ho tseba esale pele boholo ba sampole. Ha ho lintlha tse neng li qheletsoe ka thoko ho tlhahlobo; teko e ne e sa iketsahalle feela; mme bafuputsi ba ne ba tseba ka kabo nakong ea teko le tlhahlobo ea sephetho. Mehlala ea boholo bo fanoe ka litšōmo tsa litšoantšo le lifaeleng tsa data tse tala.
Nako ea poso: Apr-15-2025