Bafuputsi ba Lefapha la Biochemistry Setsing sa Saense sa India (IISc) ba sibollotse mokhoa oo e leng khale o batloabakeng sa ho laola kholo ea limela tsa khale tsa mobu tse kang bryophytes (sehlopha se kenyeletsang moss le li-liverworts) tse ileng tsa bolokoa limela tse thunyang hamorao.
Boithuto bona, bo phatlalalitsoeng koranteng ea Nature Chemical Biology, bo tsepamisitse maikutlo taolong e seng molaong ea liprotheine tsa DELLA, taolo e kholo ea kholo e hatellang karohano ea lisele ho li-embryophyte (limela tsa mobu).
Ho khahlisang, li-bryophyte, limela tsa pele tse ileng tsa hlaha mobung lilemong tse ka bang limilione tse 500 tse fetileng, ha li na moamoheli oa GID1, leha li ne li hlahisa phytohormone GA. Sena se hlahisa potso ea hore na kholo le nts'etsopele ea limela tsena tsa pele tsa mobu li ne li laoloa joang.
Ba sebelisa liverwort Marchantia polymorpha e le mokhoa oa mohlala, bafuputsi ba fumane hore limela tsena tsa khale li sebelisa enzyme e khethehileng, MpVIH, e hlahisang lenģosa la cellular inositol pyrophosphate (InsP₈), ho senya DELLA ntle le ho kenya letsoho.asiti ea gibberellik.
Bafuputsi ba sebelisitse tsamaiso ea CRISPR-Cas9 ho kokota gene e kenyang enzyme ea VIH, e tiisang karolo ea eona. Limela tse haelloang ke tšebetso ea VIH li ne li bonts'a mefokolo e matla ea nts'etsopele le ho se tloaelehe ha morphological, joalo ka makhasi a kopaneng, kholo e sa sebetseng ea radial, le khaello ea li-calyxes. Litšitiso tsena li ile tsa felisoa ka ho fetola genome ea semela ho hlahisa pheletso e le 'ngoe feela (N-terminus) ea enzyme ea VIH. E sebelisa mekhoa e tsoetseng pele ea chromatography, sehlopha se fumane hore N-terminus e na le domain kinase e susumetsang tlhahiso ea InsP₈.
Bafuputsi ba fumane hore DELLA ke e 'ngoe ea liphofu tsa cellular tsa VIH kinase. Ho feta moo, ba hlokometse hore li-phenotypes tsa limela tse haelloang ke MpVIH li ne li tšoana le tsa limela tsa M. polymorpha tse nang le polelo e eketsehileng ea DELLA.
"Nakong ena, re ne re thabetse ho utloisisa hore na botsitso kapa ts'ebetso ea DELLA e ntlafatsoa limela tse haelloang ke MpVIH," ho boletse Priyanshi Rana, sengoli sa pele le seithuti se fumaneng mangolo sehlopheng sa lipatlisiso sa Lahey. Tumellanong le maikutlo a bona, bafuputsi ba fumane hore thibelo ea DELLA e ka khutlisa haholo kholo e nang le bokooa le nts'etsopele ea phenotypes ea limela tse fetotsoeng tsa MpVIH. Liphetho tsena li fana ka maikutlo a hore VIH kinase e laola DELLA hampe, e khothalletsa kholo le nts'etsopele ea semela.
Lipatlisiso tse mabapi le liprotheine tsa DELLA li qalile nakong ea Phetohelo e Tala, ha bo-rasaense ba ne ba sebelisa monyetla oa bona ho theha mefuta e mengata ea mefuta e sa tsoaneng e nyenyane e hlahisang lihlahisoa tse ngata. Le hoja lintlha tsa mosebetsi oa bona li ne li sa hlaka ka nako eo, theknoloji ea morao-rao e lumella bo-rasaense ho laola mesebetsi ea liprotheine tsena ka boenjiniere ba liphatsa tsa lefutso, ka katleho ho eketsa chai ea lijalo.
Nako ea poso: Oct-23-2025