Bafuputsi ba Lefapha la Biochemistry Setsing sa Saense sa India (IISc) ba sibollotse mokhoa oo e leng khale o batloa o sebelisoang ke limela tsa khale tse kang bryophytes (ho kenyeletsoa mosses le li-liverworts).laola kgolo ya semela- mokhoa oo hape o bolokiloeng limela tse thunyang morao tjena.
Thuto, e hatisitsoeng koranteng ea Nature Chemical Biology, e tsepamisitse maikutlo taolong e seng ea khale ea protheine ea DELLA, molaoli o moholo oa kholo o ka thibelang karohano ea lisele ho limela tse embryonic (limela tsa mobu).
Debabrata Laha, motlatsi oa moprofesa oa biochemistry le sengoli-'moho sa thuto, ho hlalosa Debabrata Laha, "DELLA e sebetsa joalo ka lebelo, empa haeba sekhahla sena se lula se le teng, semela ha se khone ho sisinyeha." Ka hona, ho senyeha ha liprotheine tsa DELLA ho bohlokoa bakeng sa ho khothaletsa kholo ea semela. Limeleng tse thunyang, DELLA e senyeha ha phytohormonegibberellin (GA)e tlama ho moamoheli oa eona oa GID1, ho etsa motsoako oa GA-GID1-DELLA. Kamora moo, protheine e hatellang ea DELLA e tlamella liketane tsa ubiquitin mme e senngoa ke 26S proteasome.
Hoa thahasellisa hore li-bryophyte e ne e le tse ling tsa limela tsa pele tsa ho etsa kolone lilemong tse ka bang limilione tse 500 tse fetileng. Le hoja li hlahisa phytohormone gibberellin (GA), ha li na GID1 receptor. Sena se hlahisa potso: kholo le nts'etsopele ea limela tsee tsa pele tsa mobu li ne li laoloa joang?
Bafuputsi ba sebelisitse tsamaiso ea CRISPR-Cas9 ho kokota mofuta o lumellanang oa VIH, kahoo ba tiisa karolo ea VIH. Limela tse se nang enzyme ea VIH e sebetsang li bonts'a kholo e matla le mefokolo ea nts'etsopele le ho se tloaelehe ha sebopeho, joalo ka thallus e teteaneng, kholo e sa sebetseng ea radial, le khaello ea calyx. Litšitiso tsena li ile tsa lokisoa ka ho fetola genome ea semela hore e hlahise pheletso e le 'ngoe feela (N-terminus) ea enzyme ea VIH. Ho sebelisa mekhoa e tsoetseng pele ea chromatography, sehlopha sa lipatlisiso se fumane hore N-terminus e na le sebaka sa kinase se hlahisang tlhahiso ea InsP₈.
Bafuputsi ba fumane hore DELLA ke e 'ngoe ea liphofu tsa cellular tsa VIH kinase. Ho feta moo, ba hlokometse hore phenotype ea limela tse haelloang ke MpVIH e tšoana le ea limela tsa Miscanthus multiforme tse nang le polelo e eketsehileng ea DELLA.
"Mothating ona, re labalabela ho tseba hore na botsitso kapa ts'ebetso ea DELLA e ntlafatsoa limela tse haelloang ke MpVIH," ho boletse Priyanshi Rana, seithuti sa bongaka sehlopheng sa lipatlisiso sa Lahey le sengoli sa pele sa pampiri. Tumellanong le maikutlo a bona, bafuputsi ba fumane hore thibelo ea DELLA e khutlisitse haholo kholo le bofokoli ba nts'etsopele ea limela tse fetotsoeng tsa MpVIH. Liphuputso tsena li fana ka maikutlo a hore VIH kinase e laola DELLA hampe, kahoo e khothalletsa kholo le nts'etsopele ea limela.
Bafuputsi ba ile ba kopanya mekhoa ea liphatsa tsa lefutso, biochemical, le biophysical ho hlakisa mokhoa oo inositol pyrophosphate e laolang polelo ea protheine ea DELLA ho bryophyte ena. Ka ho khetheha, InsP₈, e hlahisoang ke MpVIH, e tlama ho protheine ea MpDELLA, e khothalletsang polyubiquitination ea eona, e leng se lebisang ho senyeha ha protheine ena ea khatello ka proteasome.
Lipatlisiso tse mabapi le protheine ea DELLA li qalile nakong ea Phetohelo e Tala, ha bo-ramahlale ba ne ba sebelisa monyetla oa bona ho theha mefuta e mengata e behang haholo ea semi-dwarf. Le hoja mokhoa oa eona oa ts'ebetso o ne o sa tsejoe ka nako eo, mekhoa ea morao-rao ea theknoloji e thusitse bo-rasaense ho sebelisa phetoho ea liphatsa tsa lefutso ho laola mosebetsi oa protheine ena, kahoo ba eketsa chai ea lijalo ka katleho.
"Ka keketseho ea baahi le mobu o ntseng o putlama, kotulo e ntseng e eketseha e se e le bohlokoa," ho boletse Raha. Ka lebaka la hore ho senyeha ha DELLA ho laoloang ke InsP₈ ho ka ata limeleng tse embryonic, tšibollo ena e ka bula tsela bakeng sa nts'etsopele ea lijalo tse behang haholo molokong o latelang.
Nako ea poso: Oct-31-2025