Uniconazoleke triazolemolaoli oa kholo ea limelase sebedisoang haholo ho laola bolelele ba semela le ho thibela ho hola ha peo. Leha ho le joalo, mokhoa oa limolek'hule oo uniconazole e thibelang leeding hypocotyl elongation e ntse e sa hlaka, 'me ho na le lithuto tse fokolang feela tse kopanyang data ea transcriptome le metabolome ho batlisisa mokhoa oa hypocotyl elongation. Mona, re hlokometse hore uniconazole e thibela haholo hypocotyl elongation ho lipeo tsa k'habeche tse thunyang tsa China. Hoa thahasellisa hore ho itšetlehile ka tlhahlobo e kopantsoeng ea transcriptome le metabolome, re fumane hore uniconazole e ama haholo tsela ea "phenylpropanoid biosynthesis". Tseleng ena, ke mofuta o le mong feela oa liphatsa tsa lefutso tsa taolo ea enzyme, BrPAL4, e amehang ho lignin biosynthesis, e neng e fokotsehile haholo. Ho feta moo, litekolo tsa lebasetere la tomoso e le 'ngoe le tse nyalisitsoeng tse peli li bonts'itse hore BrbZIP39 e ka ikopanya ka kotloloho le sebaka sa motšelisi sa BrPAL4 le ho kenya tšebetsong mongolo oa eona. Sistimi ea ho khutsisa liphatsa tsa lefutso e susumetsoang ke kokoana-hloko e boetse e paka hore BrbZIP39 e ka laola ka nepo ho lelefatsa ha k'habeche ea China le hypocotyl lignin synthesis. Liphetho tsa phuputso ena li fana ka leseli le lecha mabapi le mokhoa oa taolo ea limolek'hule oa cloconazole ho thibela hypocotyl elongation ea k'habeche ea China. Ho netefalitsoe lekhetlo la pele hore cloconazole e fokolitse lignin ka ho thibela motsoako oa phenylpropanoid o kentsoeng ke mojule oa BrbZIP39-BrPAL4, kahoo e lebisa ho hypocotyl dwarfing lipeong tsa k'habeche ea China.
Khabeche ea Chaena (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) ke ea mofuta oa Brassica 'me ke meroho e tsebahalang ea selemo le selemo ea cruciferous e lengoang haholo naheng ea heso (Wang et al., 2022; Yue et al., 2022). Lilemong tsa morao tjena, tekanyo ea tlhahiso ea cauliflower ea Chaena e ntse e tsoela pele ho hola, 'me mokhoa oa ho lema o fetohile ho tloha ho mokhoa oa ho jala ka ho toba ho ea ho setso se matla sa lipeo le ho jala. Leha ho le joalo, ts'ebetsong ea setso se matla sa lipeo le ho fetisoa, kholo e feteletseng ea hypocotyl e atisa ho hlahisa lipeo tse nang le maoto a maoto, e leng se bakang boleng bo bobe ba lipeo. Ka hona, ho laola kholo e feteletseng ea hypocotyl ke taba e hatellang moetlong o matla oa lipeo le ho fetisoa ha k'habeche ea China. Hona joale, ho na le liphuputso tse fokolang tse kopanyang lintlha tsa transcriptomics le metabolomics ho hlahloba mochine oa hypocotyl elongation. Mokhoa oa limolek'hule oo chlorantazole e laolang katoloso ea hypocotyl ka k'habecheng ea China ha e so ithutoe. Re ne re ikemiselitse ho tseba hore na ke liphatsa tsa lefutso life le litsela tsa limolek'hule tse arabelang ho hypocotyl dwarfing e hlahisoang ke uniconazole ka k'habecheng ea Chaena. Re sebelisa tlhahlobo ea transcriptome le metabolomic, hammoho le tlhahlobo ea lebasetere e le 'ngoe, tlhahlobo ea luciferase e habeli, le tlhahlobo e bakoang ke kokoana-hloko ea ho khutsisa (VIGS), re fumane hore uniconazole e ka etsa hore hypocotyl e fokotsehe ka har'a k'habeche ea Chaena ka ho thibela lignin biosynthesis ho peō ea k'habeche ea Chaena. Liphetho tsa rona li fana ka lintlha tse ncha mabapi le mokhoa oa taolo oa limolek'hule oo ka ona uniconazole e thibelang bolelele ba hypocotyl ka k'habecheng ea Chaena ka ho thibela phenylpropanoid biosynthesis e kentsoeng ke mojule oa BrbZIP39-BrPAL4. Liphetho tsena li ka ba le litlamorao tsa bohlokoa bakeng sa ho ntlafatsa boleng ba lipeo tsa khoebo le ho kenya letsoho ho netefatseng chai le boleng ba meroho.
BrbZIP39 ORF e bolelele bo felletseng e kentsoe ho pGreenll 62-SK ho hlahisa sephetho, 'me sekhechana sa motlatsi oa BrPAL4 se ile sa kopanngoa le lefutso la moqolotsi oa pGreenll 0800 luciferase (LUC) ho hlahisa moqolotsi oa litaba. Li-effector le liphatlalatso tsa liphatsa tsa lefutso li ile tsa fetoloa ho ba makhasi a koae (Nicotiana benthamiana).
Ho hlakisa likamano tsa metabolite le liphatsa tsa lefutso, re ile ra etsa tlhahlobo e kopaneng ea metabolome le transcriptome. Tlhahlobo ea ntlafatso ea tsela ea KEGG e bontšitse hore li-DEG le li-DAM li matlafalitsoe ka litsela tse 33 tsa KEGG (Setšoantšo sa 5A). Har'a bona, tsela ea "phenylpropanoid biosynthesis" e ne e le eona e matlafalitsoeng ka ho fetisisa; tsela ea "photosynthetic carbon fixation", tsela ea "flavonoid biosynthesis", tsela ea "pentose-glucuronic acid interconversion", tsela ea "tryptophan metabolism", le "starch-sucrose metabolism" le eona e ile ea matlafatsoa haholo. 'Mapa oa ho kopanya mocheso (Setšoantšo sa 5B) o bontšitse hore li-DAM tse amanang le li-DEG li arotsoe ka lihlopha tse' maloa, tseo har'a tsona li-flavonoids e neng e le sehlopha se seholo ka ho fetisisa, se bontšang hore tsela ea "phenylpropanoid biosynthesis" e phethile karolo ea bohlokoa ho hypocotyl dwarfism.
Bangoli ba phatlalatsa hore lipatlisiso li entsoe ho se na likamano leha e le life tsa khoebo kapa tsa lichelete tse ka nkoang e le khohlano e ka bang teng ea thahasello.
Maikutlo ohle a hlahisitsoeng sehloohong sena ke a sengoli feela 'me ha a hlile ha a bontše maikutlo a mekhatlo e ikopantseng, baphatlalatsi, bahlophisi, kapa bahlahlobisisi. Lihlahisoa life kapa life tse hlahlobiloeng sengolong sena kapa likopo tse entsoeng ke baetsi ba tsona ha li netefatsoe kapa ha li amoheloe ke mohatisi.
Nako ea poso: Mar-24-2025