Uniconazoleke triazolemolaoli oa kholo ea limelae sebediswang haholo ho laola bolelele ba semela le ho thibela ho hola ho feteletseng ha dipeo. Leha ho le jwalo, mokgwa wa dimolek'hule oo uniconazole e thibelang ho hola ha dipeo ka oona ha o so hlaka, mme ho na le dithuto tse mmalwa feela tse kopanyang data ya transcriptome le metabolome ho batlisisa mokgwa wa ho hola ha hypocotyl. Mona, re bone hore uniconazole e thibetse ho hola ha hypocotyl haholo dipeong tsa k'habeche tse thunyang tsa China. Ho thahasellisang ke hore, ho latela tlhahlobo e kopaneng ya transcriptome le metabolome, re fumane hore uniconazole e amme haholo tsela ya "phenylpropanoid biosynthesis". Tseleng ena, ke jini e le nngwe feela ya lelapa la jini le laolang enzyme, BrPAL4, e amehang ho di-biosynthesis tsa lignin, e ileng ya fokotswa haholo. Ho phaella moo, diteko tsa tomoso e le nngwe le tse pedi tse kopantsweng di bontshitse hore BrbZIP39 e ka tlama ka ho toba sebakeng sa promoter sa BrPAL4 mme ya kenya tshebetsong phetolelo ya yona. Sistimi ea ho khutsisa liphatsa tsa lefutso e bakoang ke vaerase e ile ea paka hape hore BrbZIP39 e ka laola ka nepo ho atoloha ha k'habeche ea Sechaena le ho hlophisoa ha hypocotyl lignin. Liphetho tsa phuputso ena li fana ka temohisiso e ncha mabapi le mokhoa oa taolo ea limolek'hule oa cloconazole ho thibeleng ho atoloha ha k'habeche ea Sechaena ka hypocotyl. Ho netefalitsoe ka lekhetlo la pele hore cloconazole e fokolitse litaba tsa lignin ka ho thibela ho hlophisoa ha phenylpropanoid ho tsamaisoang ke module ea BrbZIP39-BrPAL4, ka hona e lebisa ho ho nyenyefala ha hypocotyl lipeong tsa k'habeche ea Sechaena.
Khabeche ea Sechaena (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) ke ea mofuta oa Brassica 'me ke meroho e tsebahalang ea selemo le selemo ea cruciferous e lengoang haholo naheng ea heso (Wang et al., 2022; Yue et al., 2022). Lilemong tsa morao tjena, sekala sa tlhahiso ea cauliflower ea Sechaena se tsoetse pele ho hola, 'me mokhoa oa temo o fetohile ho tloha ho jaleng peo e tobileng ea setso ho ea ho jaleng peo e matla le ho e fetisetsa. Leha ho le joalo, ts'ebetsong ea ho jaleng peo e matla le ho e fetisetsa, kholo e feteletseng ea hypocotyl e atisa ho hlahisa lipeo tse maoto a malelele, e leng se fellang ka boleng bo bobe ba peo. Ka hona, ho laola kholo e feteletseng ea hypocotyl ke bothata bo boima ho jaleng peo e matla le ho e fetisetsa ho khabeche ea Sechaena. Hajoale, ho na le lithuto tse fokolang tse kopanyang data ea transcriptomics le metabolomics ho hlahloba mokhoa oa ho lelefatsa ha hypocotyl. Mokhoa oa limolek'hule oo chlorantazole e laolang katoloso ea hypocotyl ka oona ho khabeche ea Sechaena ha o so ithutoe. Re ne re ikemiseditse ho hlwaya hore na ke dijini dife le ditsela tsa dimolekhule tse arabelang ho hypocotyl dwarfing e bakwang ke uniconazole ho khabeche ya China. Re sebedisa ditshekatsheko tsa transcriptome le metabolomic, hammoho le tlhahlobo ya yeast one-hybrid, teko ya dual luciferase, le teko ya gene silencing e bakwang ke vaerase (VIGS), re fumane hore uniconazole e ka baka hypocotyl dwarfing ho khabeche ya China ka ho thibela biosynthesis ya lignin ho dipeo tsa khabeche ya China. Diphetho tsa rona di fana ka temohisiso e ntjha mabapi le mokgwa wa taolo ya dimolekhule oo uniconazole e thibelang ho lelefala ha hypocotyl ho khabeche ya China ka ho thibela biosynthesis ya phenylpropanoid e tsamaiswang ke mojule wa BrbZIP39–BrPAL4. Diphetho tsena di ka ba le ditlamorao tsa bohlokwa tse sebetsang bakeng sa ho ntlafatsa boleng ba dipeo tsa kgwebo le ho kenya letsoho ho netefatseng chai le boleng ba meroho.
BrbZIP39 ORF e bolelele bo felletseng e kentswe ka hara pGreenll 62-SK ho hlahisa effector, mme karoloana ya promoter ya BrPAL4 e ile ya kopanngwa le gene ya reporter ya pGreenll 0800 luciferase (LUC) ho hlahisa gene ya reporter. Divekthara tsa gene ya effector le reporter di ile tsa fetolwa mmoho ho ba makhasi a koae (Nicotiana benthamiana).
Ho hlakisa dikamano tsa metabolites le dijini, re entse tlhahlobo ya metabolome le transcriptome e kopaneng. Tlhahlobo ya ntlafatso ya tsela ya KEGG e bontshitse hore DEG le DAM di ne di ruisitsoe mmoho ditseleng tse 33 tsa KEGG (Setshwantsho sa 5A). Har'a tsona, tsela ya "phenylpropanoid biosynthesis" e ne e ruisitsoe haholo; tsela ya "photosynthetic carbon fixation", tsela ya "flavonoid biosynthesis", tsela ya "pentose-glucuronic acid interconversion", tsela ya "tryptophan metabolism", le tsela ya "starch-sucrose metabolism" le tsona di ne di ruisitsoe haholo. Mmapa wa ho bokella mocheso (Setshwantsho sa 5B) o bontshitse hore DAM tse amanang le DEG di ne di arotswe ka mekhahlelo e mmalwa, eo hara yona di-flavonoid e neng e le sehlopha se seholo ka ho fetisisa, e leng se bontshang hore tsela ya "phenylpropanoid biosynthesis" e bapetse karolo ya bohlokwa ho hypocotyl dwarfism.
Bangoli ba bolela hore phuputso e entsoe ntle le likamano tsa khoebo kapa tsa lichelete tse ka hlalosoang e le khohlano e ka bang teng ea lithahasello.
Maikutlo ohle a hlahisitsoeng sehloohong sena ke a mongoli feela 'me ha se hakaalo hore a bonts'a maikutlo a mekhatlo e amanang le eona, bahatisi, bahlophisi, kapa bahlahlobi. Lihlahisoa life kapa life tse hlahlobiloeng sehloohong sena kapa liqoso tse entsoeng ke bahlahisi ba tsona ha li tiisetsoe kapa ha li tšehetsoe ke mohatisi.
Nako ea poso: Hlakubele-24-2025