Mesebetsi e ka sehloohong
1. Ho kgothaletsa karohano ya sele, haholo-holo karohano ya cytoplasm;
2. Ho kgothaletsa phapang ya dikutu. Temohong ya dinama, e sebelisana le auxin ho laola phapang le ho thehwa ha metso le dikutu;
3. Ho kgothaletsa ntshetsopele ya di-bud tse ka thoko, ho fedisa puso ya apical, mme ka hona ho lebise ho thehweng ha palo e kgolo ya di-bud tse kenang ka hara setso sa dinama;
4. Ho liehisa ho tsofala ha makhasi, ho fokotsa sekhahla sa ho senyeha ha chlorophyll le liprotheine;
5. Roba peo e sa robale, nkela lesedi sebaka ho fihlela tlhoko ya lesedi ya peo e kang koae;
6. Ho kenya parthenocarpy litholoanang tse itseng;
7. Ho kgothaletsa ho thehwa ha diqalo tsa pele tsa dipalo: dipheletsong tse kgaotsweng tsa mahlaku le ho di-mosses tse ding, ho ka kgothaletsa ho thehwa ha diqalo tsa pele tsa dipalo;
8. Hlohlelletsa ho thehoa ha di-tuber tsa litapole.
E na le sebopeho sa trans feela mme e na le phello e tšoanang le eazeatin, empa ka mosebetsi o matla.
Tšusumetso ea eona e tšoana haholo le ea anti-zeatin. Ha e na feela mesebetsi ea zeatin e boletsoeng ka holimo, empa hape e na le phello ea ho kenya tšebetsong tlhahiso ea liphatsa tsa lefutso le ts'ebetso ea metabolism.
Mokhoa oa Tšebeliso
1. Ho kgothaletsa ho mela ha callus (e tlameha ho sebediswa mmoho le auxin), mahloriso a 1mg/L.
2. Khothaletsa ho beha litholoana, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, fafatsa litholoana matsatsi a 10, 25, le 40 kamora ho thunya.
3. Bakeng sa meroho e makhasi, fafatsa ka 201 mg/L ho diehisa ho ba mosehla ha makhasi.
Ho phaella moo, ho phekola peo tse ding tsa dijalo ho ka kgothaletsa ho mela ha dimela; ho phekola nakong ya ho mela ha dimela ho ka kgothaletsa kgolo.
1. Ho kgothaletsa ho mela ha dinama tse nyane (e lokela ho sebediswa mmoho le auxin), ka tekanyo ya 1 ppm;
2. Khothaletsa ho beha litholoana, 100 ppm ea cytokinin + 500 ppm ea GA3 + 20 ppm ea NAA, fafatsa litholoana matsatsi a 10, 25, le 40 kamora ho thunya;
3. Liehisa ho ba mosehla ha makhasi a meroho, fafatsa ka 20 ppm;
1. Temohong ea lisele tsa limela, mahloriso a tloaelehileng a anti-cytokinin nucleoside ke 1 mg/mL kapa ho feta.
2. Taolong ea kholo ea limela, khakanyo ea anti-cytokinin nucleoside hangata ke 1 ppm ho isa ho 100 ppm, 'me khakanyo e itseng e itšetlehile ka ts'ebeliso e itseng le mofuta oa limela. Mohlala, ha ho khothaletsoa ho mela ha lisele tsa callus, khakanyo ea anti-cytokinin nucleoside ke 1 ppm, 'me e hloka ho sebelisoa hammoho le auxin.
3. Qhibilihisa phofo ea anti-cytokinin nucleoside hantle ka 2-5 mL ea 1 M NaOH (kapa 1 M acetic acid kapa 1 M KOH), ebe u eketsa metsi a qhibilihisitsoeng habeli kapa metsi a hloekileng haholo ho lokisa tharollo ea polokelo ea 1 mg/mL kapa khatello e phahameng. Hlohlelletsa ha u ntse u eketsa metsi ho netefatsa hore e tsoakoa hantle. Tharollo ea polokelo e lokela ho aroloa 'me e hoamisoe e bolokoe ho qoba ho qhibiliha khafetsa. Fokotsa tharollo ea polokelo ka mokhoa oa temo ho fihlela khatello e hlokahalang, 'me u lokise tharollo e sebetsang hang-hang' me u e sebelise hang-hang.
Qetellong, zeatin, abscisic acid le abscisic acid nucleotide ka 'ngoe li na le litšobotsi tsa tsona mabapi le sebopeho, ts'ebetso le ts'ebeliso ea ts'ebetso. Qetellong, zeatin, abscisic acid le abscisic acid nucleotide ka 'ngoe li na le litšobotsi tsa tsona mabapi le sebopeho, ts'ebetso le ts'ebeliso ea ts'ebetso. Leha ho le joalo, kaofela li sebetsa e le balaoli ba kholo ea limela 'me li bapala karolo ea bohlokoa ho ntšetseng pele kholo le nts'etsopele ea limela.
Nako ea poso: Mphalane-22-2025