Mesebetsi e ka sehloohong
1. Khothaletsa karohano ea lisele, haholo-holo karohano ea cytoplasm;
2. Khothalletsa phapang ea li-bud. Ka setso sa lisele, e sebelisana le auxin ho laola phapang le ho thehoa ha metso le li-buds;
3. Ho khothaletsa nts'etsopele ea li-lateral buds, ho felisa puso ea apical, 'me ka hona ho lebisa ho thehoeng ha palo e kholo ea li-adventitious buds setso sa lisele;
4. Ho lieha ha makhasi a senescence, ho fokotsa sekhahla sa ho senyeha ha chlorophyll le liprotheine;
5. Hlōla boroki ba peo, fetola leseli ho fihlela litlhoko tsa leseli tse kang koae;
6. Hlohlelletsa parthenocarpy litholoana tse itseng;
7. Khothalletsa ho thehoa ha li-initials tsa li-bud: lipheletsong tse sehiloeng tsa makhasi le mosi o mong, e ka khothalletsa ho thehoa ha li-initials;
8. Khothalletsa ho thehoa ha li-tubers tsa litapole.
E na le sebopeho sa trans feela mme se na le phello e ts'oanang lezeatin, empa ka mosebetsi o matla haholoanyane.
Phello ea eona e tšoana haholo le ea anti-zeatin. Ha e na feela mesebetsi e boletsoeng ka holimo ea zeatin, empa hape e na le phello ea ho kenya tšebetsong liphatsa tsa lefutso le ts'ebetso ea metabolic.
Mokhoa oa ho Sebelisa
1. Khothalletsa ho mela ha callus (e tlameha ho sebelisoa hammoho le auxin), mahloriso 1mg/L.
2. Khothaletsa boemo ba litholoana, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, fafatsa litholoana 10, 25, le matsatsi a 40 ka mor’a ho thunya.
3. Bakeng sa meroho e makhasi, fafatsa ka 201 mg/L ho liehisa lekhasi ho ba mosehla.
Ho feta moo, ho phekola lipeo tse ling tsa lijalo ho ka khothaletsa ho mela; ho phekola sethaleng sa lipeo ho ka khothaletsa kholo.
1. Khothalletsa ho mela ha lisele tsa callus (e tlameha ho sebelisoa hammoho le auxin), ka har'a motsoako oa 1 ppm;
2. Khothalletsa ho beha litholoana, 100 ppm ea cytokinin + 500 ppm ea GA3 + 20 ppm ea NAA, fafatsa litholoana 10, 25, le matsatsi a 40 ka mor'a hore lipalesa;
3. Liehisa mosehla oa makhasi a meroho, fafatsa 20 ppm;
1. Ka setso sa lisele tsa semela, motsoako o tloaelehileng oa anti-cytokinin nucleoside ke 1 mg / mL kapa holimo.
2. Taolong ea kholo ea limela, mahloriso a anti-cytokinin nucleoside hangata ke 1 ppm ho 100 ppm, 'me mahloriso a khethehileng a itšetlehile ka kopo e khethehileng le mefuta ea limela. Ka mohlala, ha ho khothalletsa ho mela ha lisele tsa callus, motsoako oa anti-cytokinin nucleoside ke 1 ppm, 'me o hloka ho sebelisoa hammoho le auxin.
3. Hlakola phofshoana ea anti-cytokinin nucleoside ka botlalo ka 2-5 mL ea 1 M NaOH (kapa 1 M acetic acid kapa 1 M KOH), ebe u eketsa metsi a mabeli a silafetseng kapa metsi a ultrapure ho lokisetsa tharollo ea polokelo ea 1 mg / mL kapa khatello e phahameng. Hlohlelletsa ha u ntse u eketsa metsi ho etsa bonnete ba ho kopanya hantle. Tharollo ea polokelo e lokela ho tlosoa le ho hoamisoa ho bolokoa ho qoba ho qhibiliha khafetsa. Fokotsa tharollo ea polokelo ka mokhoa oa setso ho ea ho tse hlokahalang, 'me u lokise tharollo ea ho sebetsa hang-hang' me u e sebelise hang-hang.
Qetellong, zeatin, abscisic acid le abscisic acid nucleotide e 'ngoe le e' ngoe e na le litšobotsi tsa eona mabapi le sebopeho, mosebetsi le lits'ebetso tse sebetsang. Leha ho le joalo, kaofela ha tsona li sebetsa e le balaoli ba kholo ea limela 'me li phetha karolo ea bohlokoa ho khothalletsang kholo le nts'etsopele ea limela.
Nako ea poso: Oct-22-2025